Literature summary for 3.4.17.23 extracted from

Xiao, F.; Burns, K.D.
Measurement of angiotensin converting enzyme 2 activity in biological fluid (ACE2) (2017), Methods Mol. Biol., 1527, 101-115 .

Search for more literature for 3.4.17.23

Application

Application	Comment	Organism
analysis	method for measurement of ACE2 activity in biological fluids, using hydrolysis of an intramolecularly quenched fluorogenic ACE2 substrate, in the absence or presence of the ACE2 inhibitors MLN-4760 or DX600. ACE2 detection ranges from 1.56 to 50 ng/ml. MLN-4760 potently inhibits the activity of both human and mouse ACE2, DX600 (linear form) only effectively blocks human ACE2 activity in this assay. In biological samples of human and mouse urine, cell culture medium from mouse proximal tubular cells, and mouse plasma, the mean intra- and interassay coefficients of variation of the assay range from 1.43 to 4.39 %, and from 7.01 to 13.17 %, respectively	Mus musculus
analysis	method for measurement of ACE2 activity in biological fluids, using hydrolysis of an intramolecularly quenched fluorogenic ACE2 substrate, in the absence or presence of the ACE2 inhibitors MLN-4760 or DX600. ACE2 detection ranges from 1.56 to 50 ng/ml. MLN-4760 potently inhibits the activity of both human and mouse ACE2, DX600 (linear form) only effectively blocks human ACE2 activity in this assay. In biological samples of human and mouse urine, cell culture medium from mouse proximal tubular cells, and mouse plasma, the mean intra- and interassay coefficients of variation of the assay range from 1.43 to 4.39%, and from 7.01 to 13.17%, respectively	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9BYF1	-	-
Mus musculus	Q8R0I0	-	-

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Release 2023.1 (January 2023)