Cloned (Comment) | Organism |
---|---|
expression of wild-type and utant L584A ACE2 in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L584A | the point mutation in the ACE2 ectodomain markedly attenuates shedding. The resultant ACE2-L584A mutant trafficks to the cell membrane and facilitates SARS-CoV entry into target cells | Homo sapiens |
additional information | construction of cytoplasmic tail deletion mutants by introduction of a stop codon at position amino acid 763. Construction of chimeric proteins containing portions of human ACE2 and portions of human CD4 or human beta-defensin-2, both showing loss of domain shedding | Homo sapiens |
N580A | the mutation in the ectodomain has no effect on sACE2 release | Homo sapiens |
P583A | the mutation in the ectodomain has no effect on sACE2 release | Homo sapiens |
R582A | the mutation in the ectodomain has no effect on sACE2 release | Homo sapiens |
V581A | the mutation in the ectodomain has no effect on sACE2 release | Homo sapiens |
V604A | the mutation in the ectodomain has no effect on sACE2 release | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
membrane | ACE 2 is shedded | Homo sapiens | 16020 | - |
soluble | ACE 2 is shedded | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | ACE2 is a terminal carboxypeptidase and the receptor for the SARS and NL63 coronaviruses. Soluble sACE2 acts as receptor binding SARS-CoV glycoprotein S pseudotyped FIV virus and blocks virus infection of target cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BYF1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | ACE2 is is shed from human airway epithelia, constitutive generation of soluble ACE2 is inhibited by ADAM17 inhibitor DPC 333, i.e. (2R)-2-[(3R)-3-amino-3(4-[2-methyl-(4-quinolinyl) methoxy] phenyl)-2-oxopyrrolidinyl]-N-hydroxy-4-methylpentanamide, but not by while ADAM10 inhibitor GI254023, while phorbol ester, ionomycin, endotoxin, and IL-1beta and TNFalpha acutely induce ACE2 release, thus, the regulation of ACE2 cleavage involves a disintegrin and metalloprotease 17, ADAM17, and ADAM10, overview. The ACE2 ectodomain regulates its release and residue L584 might be part of a putative sheddase recognition motif | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bronchoalveolar lavage fluid | - |
Homo sapiens | - |
Calu-3 cell | - |
Homo sapiens | - |
epithelium | from airway, apical surface | Homo sapiens | - |
HT-1080 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ACE2 is a terminal carboxypeptidase and the receptor for the SARS and NL63 coronaviruses. Soluble sACE2 acts as receptor binding SARS-CoV glycoprotein S pseudotyped FIV virus and blocks virus infection of target cells | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the membraneous enzyme contains an ectodomain which is cleaved in the shedding process resulting in the stille active soluble enzyme form, regulation, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ACE2 | - |
Homo sapiens |
angiotensin converting enzyme 2 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of ACE2 function is implicated in severe acute respiratory syndrome, SARS, pathogenesis | Homo sapiens |
physiological function | soluble sACE2 may play a role in modifying peptides in airway surface liquid involved in processes such as inflammation. The membrane-associated form of ACE2 serves as a SARS-CoV receptor in vitro, and shedding is not required for infection to occur | Homo sapiens |