Cloned (Comment) | Organism |
---|---|
expression in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K481Q | angiotensin I cleavage activity is 21% of wild-type activity, angiotensin II cleavage activity is 71.8% of wild-type activity | Homo sapiens |
R169Q | angiotensin I cleavage activity is 5.2% of wild-type activity, angiotensin II cleavage activity is 1.1% of wild-type activity. The mutant enzyme does not show any activity with angiotensin I in the absence of chloride ions | Homo sapiens |
R169QK481QR514Q | angiotensin I cleavage activity is 53.2% of wild-type activity, angiotensin II cleavage activity is 203.4% of wild-type activity | Homo sapiens |
R514Q | angiotensin I cleavage activity is 52% of wild-type activity, angiotensin II cleavage activity is 179.3% of wild-type activity, enhancement of angiotensin II cleavage is a result of a 2.5-fold increase in Vmax compared with the wild-type | Homo sapiens |
W271A | angiotensin I cleavage activity is 5.3% of wild-type activity, angiotensin II cleavage activity is 0.9% of wild-type activity. Lacks any significant chloride sensitivity with the substrate angiotensin I | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | ACE2 activity is regulated by chloride ions. The presence of chloride increases the hydrolysis of angiotensin I by ACE2, but inhibits cleavage of the vasoconstrictor angiotensin II | Homo sapiens | |
MLN-4760 | ACE2-specific inhibitor. Inhibition of wild-type ACE2 was sensitive to chloride concentration | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
angiotensin II | pH 7.4, 37°C, mutant enzyme R514Q | Homo sapiens | |
0.0586 | - |
angiotensin II | pH 7.4, 37°C, wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | ACE2 activity is regulated by chloride ions. The presence of chloride increases the hydrolysis of angiotensin I by ACE2, but inhibits cleavage of the vasoconstrictor angiotensin II | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BYF1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | - |
Homo sapiens | angiotensin-(1-9) + Leu | - |
? | |
angiotensin II + H2O | - |
Homo sapiens | angiotensin-(1-7) + Phe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACE2 | - |
Homo sapiens |