Literature summary for 3.4.17.23 extracted from

Guy, J.L.; Jackson, R.M.; Acharya, K.R.; Sturrock, E.D.; Hooper, N.M.; Turner, A.J.
Angiotensin-converting enzyme-2 (ACE2): comparative modeling of the active site, specificity requirements, and chloride dependence (2003), Biochemistry, 42, 13185-13192.

Search for more literature for 3.4.17.23

Application

Application	Comment	Organism
medicine	potential important target in cardio-renal disease	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the mutant enzyme in CHO cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a soluble truncated mutant enzyme lacking the transmembrane and cytosolic domains	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Cl-	inhibition is substrate dependent, inhibitory with substrate angiotensin II	Homo sapiens
additional information	no inhibition by lisinopril	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	angiotensin II	pH 7.4, 37°C	Homo sapiens
0.0091	-	angiotensin 3-8	pH 7.4, 37°C	Homo sapiens
0.0126	-	angiotensin 4-8	pH 7.4, 37°C	Homo sapiens
0.0245	-	angiotensin 5-8	pH 7.4, 37°C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cl-	binding ligands are Tyr207 and Arg514, possible model for chloride activation, effect is substrate dependent: activation with angiotensin I and (7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH, inhibition with angiotensin II	Homo sapiens
Zn2+	zinc-binding motif HEXXH and third zinc ligand glutamate402, zinc-binding protease	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
angiotensin I + H2O	Homo sapiens	-	angiotensin-(1-9) + Leu	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9BYF1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH + H2O	synthetic fluorogenic substrate	Homo sapiens	(7-methoxycoumarin-4-yl)acetyl-AP + N6-(2,4-dinitrophenyl)-L-lysine	-	?
angiotensin I + H2O	-	Homo sapiens	angiotensin-(1-9) + Leu	-	?
angiotensin II + H2O	-	Homo sapiens	angiotensin-(1-7) + Phe	-	ir
angiotensin-(3-8) + H2O	-	Homo sapiens	angiotensin-(3-7) + Phe	-	ir
angiotensin-(4-8) + H2O	-	Homo sapiens	angiotensin-(4-7) + Phe	-	ir
angiotensin-(5-8) + H2O	-	Homo sapiens	angiotensin-(5-7) + Phe	-	ir

Synonyms

Synonyms	Comment	Organism
ACE2	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
84	-	angiotensin 4-8	pH 7.4, 37°C	Homo sapiens
162	-	angiotensin 3-8	pH 7.4, 37°C	Homo sapiens
1110	-	angiotensin II	pH 7.4, 37°C	Homo sapiens
1518	-	angiotensin 5-8	pH 7.4, 37°C	Homo sapiens

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Release 2023.1 (January 2023)