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Literature summary for 2.4.2.6 extracted from

  • Fresco-Taboada, A.; Serra, I.; Fernandez-Lucas, J.; Acebal, C.; Arroyo, M.; Terreni, M.; de la Mata, I.
    Nucleoside 2'-deoxyribosyltransferase from psychrophilic bacterium Bacillus psychrosaccharolyticus - preparation of an immobilized biocatalyst for the enzymatic synthesis of therapeutic nucleosides (2014), Molecules, 19, 11231-11249.
    View publication on PubMed
Search for more literature for 2.4.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Peribacillus psychrosaccharolyticus

General Stability

General Stability Organism
the enzyme is stable when immobilized on PEI-agarose but shows no activity when immobilized on glyoxyl-agarose. The best stability is obtained using dextrane oxidized at 70% (w/v) Peribacillus psychrosaccharolyticus

Inhibitors

Inhibitors Comment Organism Structure
NaCl activity decreases 25% in presence of 1.5 M NaCl Peribacillus psychrosaccharolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Na+
-
 Peribacillus psychrosaccharolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 6 * 16000, SDS-PAGE Peribacillus psychrosaccharolyticus
91400
-
 gel filtration Peribacillus psychrosaccharolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-deoxy-D-ribosyl-base1 + base2 Peribacillus psychrosaccharolyticus
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2 Peribacillus psychrosaccharolyticus CECT 4074
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?

Organism

Organism UniProt Comment Textmining
Peribacillus psychrosaccharolyticus
-
-
-
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration Peribacillus psychrosaccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyuridine + 5-trifluorothymine
-
 Peribacillus psychrosaccharolyticus 5-trifluorothymidine + uracil
-
 ?
2'-deoxyuridine + 5-trifluorothymine
-
 Peribacillus psychrosaccharolyticus CECT 4074 5-trifluorothymidine + uracil
-
 ?
2'-deoxyuridine + adenine
-
 Peribacillus psychrosaccharolyticus 2'-deoxyadenosine + uracil
-
 ?
2'-deoxyuridine + adenine
-
 Peribacillus psychrosaccharolyticus CECT 4074 2'-deoxyadenosine + uracil
-
 ?
2'-deoxyuridine + thymidine
-
 Peribacillus psychrosaccharolyticus 2'-deoxythymidine + uracil
-
 ?
2'-deoxyuridine + thymidine
-
 Peribacillus psychrosaccharolyticus CECT 4074 2'-deoxythymidine + uracil
-
 ?
2-deoxy-D-ribosyl-base1 + base2
-
 Peribacillus psychrosaccharolyticus 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2-deoxy-D-ribosyl-base1 + base2
-
 Peribacillus psychrosaccharolyticus CECT 4074 2-deoxy-D-ribosyl-base2 + base1
-
 ?
additional information the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces Peribacillus psychrosaccharolyticus ?
-
 ?
additional information the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces Peribacillus psychrosaccharolyticus CECT 4074 ?
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 16000, SDS-PAGE Peribacillus psychrosaccharolyticus

Synonyms

Synonyms Comment Organism
NDT
-
 Peribacillus psychrosaccharolyticus
nucleoside 2'-deoxyribosyltransferase
-
 Peribacillus psychrosaccharolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Peribacillus psychrosaccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 90 the enzyme is stable up to 50°C during 15 min Peribacillus psychrosaccharolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Peribacillus psychrosaccharolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 the enzyme is stable in a pH range from 6.0 to 9.0, and retains more than 85% activity at pH 10.0 Peribacillus psychrosaccharolyticus

pI Value

Organism Comment pI Value Maximum pI Value
Peribacillus psychrosaccharolyticus isoelectric focusing
-
 4.6