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Literature summary for 2.1.3.6 extracted from

  • Stalon, V.
    Putrescine carbamoyltransferase (Streptococcus faecalis) (1983), Methods Enzymol., 94, 339343.
No PubMed abstract available
Search for more literature for 2.1.3.6

Inhibitors

Inhibitors Comment Organism Structure
4-aminobutyrate
-
 Enterococcus faecalis
arginine
-
 Enterococcus faecalis
arsenate competitive vs. carbamoylphosphate Enterococcus faecalis
diphosphate competitive vs. carbamoylphosphate Enterococcus faecalis
glutamate
-
 Enterococcus faecalis
guanidobutyrate
-
 Enterococcus faecalis
norvaline
-
 Enterococcus faecalis
Oxalurate
-
 Enterococcus faecalis
phosphate competitive vs. carbamoylphosphate Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 putrescine at pH 7.0 Enterococcus faecalis
0.17
-
 Carbamoyl phosphate at pH 7.0 Enterococcus faecalis
4
-
 arsenate arsenolytic cleavage Enterococcus faecalis
10
-
 N-Carbamoylputrescine arsenolytic cleavage Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 3 * 40000, treatment with glutaraldehyde, SDS-PAGE Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Pediococcus acidilactici
-
-
-

Purification (Commentary)

Purification (Comment) Organism
65°C, ammonium sulfate, DEAE-Sephadex, Sephadex G-200 Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
460
-
-
 Enterococcus faecalis

Storage Stability

Storage Stability Organism
-20°C, 2.5 mg/ml, 50 mM potassium phosphate, pH 7.5, 3 years, no loss of activity Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenate + N-carbamoylputrescine
-
 Enterococcus faecalis ?
-
 ?
carbamoyl phosphate + 1,3-diaminopropane
-
 Enterococcus faecalis phosphate + N1-carbamoyl-1,3-diaminopropane
-
 ?
carbamoyl phosphate + 1,6-diaminohexane
-
 Enterococcus faecalis phosphate + N1-carbamoyl-1,6-diaminohexane
-
 ?
carbamoyl phosphate + cadaverine
-
 Enterococcus faecalis phosphate + N-carbamoylcadaverine
-
 ?
carbamoyl phosphate + ornithine
-
 Enterococcus faecalis phosphate + citrulline
-
 ?
carbamoyl phosphate + putrescine
-
 Enterococcus faecalis phosphate + N-carbamoylputrescine
-
 ?
carbamoyl phosphate + spermidine
-
 Enterococcus faecalis phosphate + N-carbamoylspermidine
-
 ?
carbamoyl phosphate + spermine
-
 Enterococcus faecalis phosphate + N-carbamoylspermine
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 40000, treatment with glutaraldehyde, SDS-PAGE Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 at 10 mM putrescine and carbamoyl phosphate Enterococcus faecalis
7.8
-
 at at 0.5 mM putrescine and carbamyol phosphate Enterococcus faecalis
9
-
 at 0.5 mM putrescine Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
 diphosphate
-
 Enterococcus faecalis
4
-
 phosphate
-
 Enterococcus faecalis
12
-
 arsenate
-
 Enterococcus faecalis