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Literature summary for 2.1.1.57 extracted from

  • Stewart, M.E.; Roy, P.
    Structure-based identification of functional residues in the nucleoside-2-O-methylase domain of Bluetongue virus VP4 capping enzyme (2015), FEBS open bio, 5, 138-146.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
baculovirus expression system bluetongue virus

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.5 A resolution. Residues 175 to 377 encode a distinct 2'-O MT domain segregated from the other domains and responsible for methylation of cap0 structure bluetongue virus

Protein Variants

Protein Variants Comment Organism
D265E complete loss of 2'-O-methylation activity, Bluetongue virus replication in cells is abrogated bluetongue virus
D265V complete loss of 2'-O-methylation activity, Bluetongue virus replication in cells is abrogated bluetongue virus
N311A 2fold reduction of 2'-O-methylation activity, Bluetongue virus replication in cells is abrogated bluetongue virus
N311A/Y334/R367A 2fold reduction of 2'-O-methylation activity, Bluetongue virus replication in cells is abrogated bluetongue virus
R367A mutation does not affect methyl group transfer bluetongue virus
Y334A mutation does not affect methyl group transfer bluetongue virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
x * 74000, calculated bluetongue virus

Organism

Organism UniProt Comment Textmining
bluetongue virus C5IWW0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7Me-GpppG
-
bluetongue virus S-adenosyl-L-homocysteine + 7Me-Gppp-2'OMe-G
-
?

Subunits

Subunits Comment Organism
? x * 74000, calculated bluetongue virus

Synonyms

Synonyms Comment Organism
VP4
-
bluetongue virus