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Literature summary for 2.1.1.57 extracted from

  • Ke, M.; Chen, Y.; Wu, A.; Sun, Y.; Su, C.; Wu, H.; Jin, X.; Tao, J.; Wang, Y.; Ma, X.; Pan, J.A.; Guo, D.
    Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2'-O-methyltransferase activity of nsp10/nsp16 complex (2012), Virus Res., 167, 322-328.
    View publication on PubMed
Search for more literature for 2.1.1.57

Activating Compound

Activating Compound Comment Organism Structure
nsp10 protein nsp10 acts as an essential trigger to activate the 2'-O-methyltransferase activity of nsp16. The region of amino acids 42–120 in nsp10 is needed for stimulating the nsp16 2'-O-methyltransferase activity Severe acute respiratory syndrome-related coronavirus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Severe acute respiratory syndrome-related coronavirus

Inhibitors

Inhibitors Comment Organism Structure
additional information peptides K12 (aa 68-80 of nsp10), and K29 (aa 68-96 of nsp10) decrease the S-adenosyl-L-methionine (SAM) binding by nsp16 in a dose-dependent manner. When 0.4 mM of K29 is used, it almost abolishes the SAM-binding activity of nsp16. In contrast, the peptides K8 (aa 100-107 of nsp10), K10 (aa 91-100 of nsp10) and K20 (aa 40-59 of nsp10), which cannot inhibit the 2'-O-MTase activity of nsp16, have no effect on SAM binding of nsp16, even when the final concentration of 0.5 mM is applied Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
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Severe acute respiratory syndrome-related coronavirus WHU
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-
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Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + m7G(5')pppA-RNA
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 Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7G(5')pppAm-RNA
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 ?
S-adenosyl-L-methionine + m7G(5')pppA-RNA
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 Severe acute respiratory syndrome-related coronavirus WHU S-adenosyl-L-homocysteine + m7G(5')pppAm-RNA
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 ?

Synonyms

Synonyms Comment Organism
2'-O-methyltransferase
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 Severe acute respiratory syndrome-related coronavirus
2'-O-MTase
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 Severe acute respiratory syndrome-related coronavirus
2'O-MTase
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 Severe acute respiratory syndrome-related coronavirus
nsp10/nsp16 complex
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 Severe acute respiratory syndrome-related coronavirus
RNA cap 2'-O-MTase
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 Severe acute respiratory syndrome-related coronavirus