Literature summary for 2.1.1.57 extracted from

Decroly, E.; Debarnot, C.; Ferron, F.; Bouvet, M.; Coutard, B.; Imbert, I.; Gluais, L.; Papageorgiou, N.; Sharff, A.; Bricogne, G.; Ortiz-Lombardia, M.; Lescar, J.; Canard, B.
Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2�-O-methyltransferase nsp10/nsp16 complex (2011), PLoS Pathog., 7, e1002059.

Activating Compound

Activating Compound	Comment	Organism	Structure
nsp10 protein	the methyltransferase is only active in the presence of its activating partner nsp10	Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants	Comment	Organism
D106A	the mutant shows 38% of wild type activity	Severe acute respiratory syndrome-related coronavirus
D130A	the mutant shows 2% of wild type activity	Severe acute respiratory syndrome-related coronavirus
D99A	inactive	Severe acute respiratory syndrome-related coronavirus
E203A	inactive	Severe acute respiratory syndrome-related coronavirus
G73A	the mutant shows 18% of wild type activity	Severe acute respiratory syndrome-related coronavirus
I40A	the mutant shows 8% of wild type activity	Severe acute respiratory syndrome-related coronavirus
K170A	inactive	Severe acute respiratory syndrome-related coronavirus
K46A	the mutant shows 1% of wild type activity	Severe acute respiratory syndrome-related coronavirus
L244A	inactive	Severe acute respiratory syndrome-related coronavirus
M247A	inactive	Severe acute respiratory syndrome-related coronavirus
M41A	the mutant shows 4% of wild type activity	Severe acute respiratory syndrome-related coronavirus
N43A	the mutant shows 11% of wild type activity	Severe acute respiratory syndrome-related coronavirus
Q87A	the mutant shows 62% of wild type activity	Severe acute respiratory syndrome-related coronavirus
R86A	inactive	Severe acute respiratory syndrome-related coronavirus
S188A	the substitution shows 72% loss of activity with no significant effect on the stability of the nsp10/nsp16 complex	Severe acute respiratory syndrome-related coronavirus
T48A	the mutant shows 20% of wild type activity	Severe acute respiratory syndrome-related coronavirus
T54E	the substitution shows 72% loss of activity with no significant effect on the stability of the nsp10/nsp16 complex	Severe acute respiratory syndrome-related coronavirus
T58A	the substitution shows 43% loss of activity with no significant effect on the stability of the nsp10/nsp16 complex	Severe acute respiratory syndrome-related coronavirus
T58E	the substitution shows 99% loss of activity with 54% association of the Nsp10/nsp16 complex compared to the wild type	Severe acute respiratory syndrome-related coronavirus
T58N	the substitution shows 70% loss of activity with no significant effect on the stability of the nsp10/nsp16 complex	Severe acute respiratory syndrome-related coronavirus
V104G	the mutant shows 4% of wild type activity	Severe acute respiratory syndrome-related coronavirus
V44A	the mutant shows 1% of wild type activity	Severe acute respiratory syndrome-related coronavirus
V78A	inactive	Severe acute respiratory syndrome-related coronavirus
Y132A	the mutant shows 5% of wild type activity	Severe acute respiratory syndrome-related coronavirus
Y132F	the mutant shows 9% of wild type activity	Severe acute respiratory syndrome-related coronavirus
Y132H	inactive	Severe acute respiratory syndrome-related coronavirus
Y132T	the mutant shows 5% of wild type activity	Severe acute respiratory syndrome-related coronavirus
Y30A	the mutant shows 1% of wild type activity	Severe acute respiratory syndrome-related coronavirus
Y30F	the mutant shows 6% of wild type activity	Severe acute respiratory syndrome-related coronavirus
Y96A	the mutation abrogates nsp10/nsp16 interaction with enzyme activity	Severe acute respiratory syndrome-related coronavirus
Y96F	the mutation increases both nsp10/nsp16 interaction with MTase activity and enzyme activity	Severe acute respiratory syndrome-related coronavirus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	contains Mg2+, Mg2+ can be substituted by Mn2+ or Ca2+, but not Zn2+	Severe acute respiratory syndrome-related coronavirus

Organism

Organism	UniProt	Comment	Textmining
Severe acute respiratory syndrome-related coronavirus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + m7G(5')pppA-RNA	-	Severe acute respiratory syndrome-related coronavirus	S-adenosyl-L-homocysteine + m7G(5')pppAm-RNA	-	?

Synonyms

Synonyms	Comment	Organism
(nucleoside-2'-O-)-methyltransferase	-	Severe acute respiratory syndrome-related coronavirus
2'-O-MTase	-	Severe acute respiratory syndrome-related coronavirus
nsp10/16 complex	-	Severe acute respiratory syndrome-related coronavirus
nsp16 protein	-	Severe acute respiratory syndrome-related coronavirus
RNA cap 2'-O-methyltransferase	-	Severe acute respiratory syndrome-related coronavirus