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Literature summary for 2.1.1.57 extracted from

  • Lugari, A.; Betzi, S.; Decroly, E.; Bonnaud, E.; Hermant, A.; Guillemot, J.C.; Debarnot, C.; Borg, J.P.; Bouvet, M.; Canard, B.; Morelli, X.; Lecine, P.
    Molecular mapping of the RNA cap 2'-O-methyltransferase activation interface between severe acute respiratory syndrome coronavirus nsp10 and nsp16 (2010), J. Biol. Chem., 285, 33230-33241.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
nsp10 protein nsp16 carries RNA cap 2'-O-methyltransferase activity only in the presence of its interacting partner nsp10 Severe acute respiratory syndrome-related coronavirus

Application

Application Comment Organism
medicine the nsp10-nsp16 interface represents an attractive target for antivirals against human and animal pathogenic coronaviruses Severe acute respiratory syndrome-related coronavirus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 (DE3) cells Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
G70A the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
G94A the nsp10 mutation binds nsp16 with a slightly reduced affinity (60%) but is still able to stimulate nsp16 2'O-MTase activity Severe acute respiratory syndrome-related coronavirus
G94D the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
K93A the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
K93E the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
K95A the nsp10 mutation binds nsp16 with a slightly reduced affinity (80%) but is still able to stimulate nsp16 2'O-MTase activity Severe acute respiratory syndrome-related coronavirus
M44A the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
R78A the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
R78G the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
S72A the mutant protein binds nsp16 but weakly activates 2'O-MTase activity Severe acute respiratory syndrome-related coronavirus
V42A the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
Y96A the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
Y96F the nsp10 mutation overstimulates nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
Y96I the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus
Y96V the nsp10 mutation abrogates stimulation of nsp16 enzyme activity Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisPur cobalt resin column chromatography Severe acute respiratory syndrome-related coronavirus

Synonyms

Synonyms Comment Organism
2'-O-methyltransferase
-
Severe acute respiratory syndrome-related coronavirus
2'-O-MTase
-
Severe acute respiratory syndrome-related coronavirus
nsp16
-
Severe acute respiratory syndrome-related coronavirus
RNA cap 2'-O-methyltransferase
-
Severe acute respiratory syndrome-related coronavirus
RNA cap 2'O-MTase
-
Severe acute respiratory syndrome-related coronavirus