Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nsp10 protein | nsp16 carries RNA cap 2'-O-methyltransferase activity only in the presence of its interacting partner nsp10 | Severe acute respiratory syndrome-related coronavirus |
Application | Comment | Organism |
---|---|---|
medicine | the nsp10-nsp16 interface represents an attractive target for antivirals against human and animal pathogenic coronaviruses | Severe acute respiratory syndrome-related coronavirus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41 (DE3) cells | Severe acute respiratory syndrome-related coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
G70A | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
G94A | the nsp10 mutation binds nsp16 with a slightly reduced affinity (60%) but is still able to stimulate nsp16 2'O-MTase activity | Severe acute respiratory syndrome-related coronavirus |
G94D | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
K93A | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
K93E | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
K95A | the nsp10 mutation binds nsp16 with a slightly reduced affinity (80%) but is still able to stimulate nsp16 2'O-MTase activity | Severe acute respiratory syndrome-related coronavirus |
M44A | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
R78A | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
R78G | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
S72A | the mutant protein binds nsp16 but weakly activates 2'O-MTase activity | Severe acute respiratory syndrome-related coronavirus |
V42A | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
Y96A | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
Y96F | the nsp10 mutation overstimulates nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
Y96I | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
Y96V | the nsp10 mutation abrogates stimulation of nsp16 enzyme activity | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisPur cobalt resin column chromatography | Severe acute respiratory syndrome-related coronavirus |
Synonyms | Comment | Organism |
---|---|---|
2'-O-methyltransferase | - |
Severe acute respiratory syndrome-related coronavirus |
2'-O-MTase | - |
Severe acute respiratory syndrome-related coronavirus |
nsp16 | - |
Severe acute respiratory syndrome-related coronavirus |
RNA cap 2'-O-methyltransferase | - |
Severe acute respiratory syndrome-related coronavirus |
RNA cap 2'O-MTase | - |
Severe acute respiratory syndrome-related coronavirus |