Literature summary for 2.1.1.57 extracted from

Mastrangelo, E.; Bollati, M.; Milani, M.; de Lamballeire, X.; Brisbare, N.; Dalle, K.; Lantez, V.; Egloff, M.P.; Coutard, B.; Canard, B.; Gould, E.; Forrester, N.; Bolognesi, M.
Preliminary characterization of (nucleoside-2-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses (2006), Acta Crystallogr. Sect. F, F62, 768-770.

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Application

Application	Comment	Organism
analysis	sequence homology relative to Dengue virus 2'OMTase	Meaban virus
analysis	sequence homology relative to Dengue virus 2'OMTase	Yokose virus

Cloned(Commentary)

Cloned (Comment)	Organism
as N-terminal His6-tag expressed in a pDEST 14 HN expression vector and transformed into Escherichia coli Rosetta (DE3) pRos	Meaban virus
as N-terminal His6-tag expressed in a pDEST 14 HN expression vector and transformed into Escherichia coli Rosetta (DE3) pRos	Yokose virus

Crystallization (Commentary)

Crystallization (Comment)	Organism
by vapour-diffusion, to 2.7 A resolution, in complex with S-adenosyl-l-methionine, crystals belong to the orthorhombic space group P21212, with unit-cell parameters a = 58.7, b = 126.6, c = 156.1 A, presence of three MTase molecules in the asymmetric unit, with a solvent content of 57.7%, residues Lys62, Asp147, Lys184 and Glu220 specifically characterize the active-site region	Yokose virus
by vapour-diffusion, to 2.8 A resolution, in complex with S-adenosyl-l-methionine, crystals belong to the tetragonal space group P43, with unit-cell parameters a = b = 83.0, c = 170.2 A, presence of four MTase molecules per asymmetric unit and a corresponding solvent content of 49.9%, residues Lys62, Asp147, Lys184 and Glu220 specifically characterize the active-site region	Meaban virus

Organism

Organism	UniProt	Comment	Textmining
Meaban virus	-	-	-
Yokose virus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration	Meaban virus
by gel filtration	Yokose virus

Synonyms

Synonyms	Comment	Organism
(nucleoside-2'-O-)-methyltransferase	-	Meaban virus
(nucleoside-2'-O-)-methyltransferase	-	Yokose virus
2'OMTases	-	Meaban virus
2'OMTases	-	Yokose virus

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Release 2023.1 (January 2023)