Literature summary for 2.1.1.57 extracted from

Schnierle, B.S.; Gershon, P.D.; Moss, B.
Mutational analysis of a multifunctional protein, with mRNA 5' cap-specific (nucleoside-2'-O-)-methyltransferase and 3'-adenylyltransferase stimulatory activities, encoded by vaccinia virus (1994), J. Biol. Chem., 269, 20700-20706.

Search for more literature for 2.1.1.57

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type, exchange mutants and open-reading-frame mutant in Escherichia coli, all glutathione-S-transferase-tagged	Vaccinia virus

Protein Variants

Protein Variants	Comment	Organism
C178S	point mutations, glutathione-S-transferase-tagged, exchange shows no effect	Vaccinia virus
C272S	point mutations, glutathione-S-transferase-tagged, exchange shows no effect	Vaccinia virus
additional information	333-codon open-reading frame is cut out and expressed in Escherichia coli, reduced methyltransferase and RNA binding activity	Vaccinia virus
additional information	charged amino acids Asp, Glu, His, Lys, Arg are exchanged for Ala by oligonucleotide-directed mutagenesis, glutathione-S-transferase-tagged, 11 of 21 Ala-mutants show reduced activity, 4 show increased activity	Vaccinia virus

Organism

Organism	UniProt	Comment	Textmining
Vaccinia virus	-	VP39 protein	-

Purification (Commentary)

Purification (Comment)	Organism
fragment mutant and exchange mutants from recombinant E. coli	Vaccinia virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + m7G(5')pppR-RNA	-	Vaccinia virus	S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA	-	?

Synonyms

Synonyms	Comment	Organism
mRNA 5' cap-specific (nucleoside-2'-O)-methyltransferase	i.e. VP39	Vaccinia virus

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Release 2023.1 (January 2023)