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Literature summary for 2.1.1.57 extracted from

  • Egloff, M.P.; Benarroch, D.; Selisko, B.; Romette, J.L.; Canard, B.
    An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization (2002), EMBO J., 21, 2757-2768.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.57

Cloned(Commentary)

Cloned (Comment) Organism
cloning of His-tagged N-terminal part of NS5 protein, termed NS5MTaseDV, amino acids 1-296, overexpression in Escherichia coli Dengue virus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method at room temperature, 0.1 M sodium citrate pH 5.8, 1.2 M lithium sulfate, 0.5 M ammonium sulfate, vapour diffusion for 1 week, structure analysis with and without bound nucleotide, model Dengue virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 recombinant truncated N-terminal protein, gel filtration Dengue virus

Organism

Organism UniProt Comment Textmining
Dengue virus P12823 type 2, NS5 protein
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged N-terminal part of NS5 protein from E. coli Dengue virus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA] nucleotide binding analysis based on crystal structure Dengue virus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + GpppACCCCC
-
 Dengue virus S-adenosyl-L-homocysteine + GpppAmCCCCC
-
 ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA His-tagged N-terminal part of NS5 protein, termed NS5MTaseDV, amino acids 1-296, utilizes methylated and unmethylated capped RNA, but not uncapped RNA Dengue virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
 ?
S-adenosyl-L-methionine + m7GpppACCCCC
-
 Dengue virus S-adenosyl-L-homocysteine + m7GpppAmCCCCC
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, recombinant truncated N-terminal protein, gel filtration, SDS-PAGE Dengue virus

Synonyms

Synonyms Comment Organism
mRNA cap (nucleoside-2'-O)-methyltransferase i.e. NS5 Dengue virus