Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | cap I-methyltransferase; strong | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000014 | - |
G(5')pppA-poly(A) | - |
Homo sapiens | |
0.0000016 | - |
m7G(5')pppA-poly(A) | - |
Homo sapiens | |
0.0006 | - |
S-adenosyl-L-methionine | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | cap I- and cap II-methyltransferase | Homo sapiens | 5737 | - |
nucleus | cap I-methyltransferase | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activation, 140-180 mM, cap I-methyltransferase | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HeLa-cells, phosphocellulose chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + G(5')pppA-poly(A) | - |
Homo sapiens | S-adenosyl-L-homocysteine + G(5')pppA-2'-OMe-poly(A) | - |
r | |
S-adenosyl-L-methionine + m7G(5')pppA-poly(A) | - |
Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppA-2'-OMe-poly(A) | - |
r | |
S-adenosyl-L-methionine + m7G(5')pppNmpN-RNA | N: purin and pyrimidine nucleotides are methylated, enzyme: cap II-methyltransferase | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppNmpNm-RNA | i.e. cap II | ? | |
S-adenosyl-L-methionine + m7G(5')pppNpN-RNA | i.e. cap O, N: purine and pyrimidine nucleotides are methylated, enzyme: cap I-methyltransferase, substrates are RNAs with a capped terminus with at least 2 additional nucleotides, G(5')pppNpNp | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppNmpN-RNA | i.e. cap I | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | satellite tobacco necrosis virus | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | unmethylated vaccinia virus mRNA | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | best substrates are poly(A)-nucleosides and poly-(I)-nucleosides with m7G(5')pppN-ends | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? | |
S-adenosyl-L-methionine + m7GpppApGp | i.e. capped dinucleotide, cap I-methyltransferase | Homo sapiens | S-adenosyl-L-homocysteine + m7GpppAmpGp | - |
? | |
S-adenosyl-L-methionine + m7GpppApGpUp | i.e. capped trinucleotide, cap I-methyltransferase | Homo sapiens | S-adenosyl-L-homocysteine + m7GpppAmpGpUp | - |
? | |
S-adenosyl-L-methionine + mononucleotide | such as m7GpppA or GpppA, poor substrates for cap I-methyltransferase | Homo sapiens | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
10 min stable | Homo sapiens |
44 | - |
and above, inactivation after 20 min | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
beginning from pH 6.9 continous increase of activity | Homo sapiens |