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Literature summary for 2.1.1.57 extracted from

  • Barbosa, E.; Moss, B.
    mRNA(nucleoside-2-)-methyltransferase from vaccinia virus. Characteristics and substrate specificity (1978), J. Biol. Chem., 253, 7698-7702.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin stabilizes Vaccinia virus
glycerol, 10% v/v stabilizes Vaccinia virus

Inhibitors

Inhibitors Comment Organism Structure
7-methylguanosine(5')pppN-dinucleotides weak, competitive Vaccinia virus
additional information no inhibition by poly(A), poly(U), poly(C) or poly(G) Vaccinia virus
NaCl above 0.05 M Vaccinia virus
S-adenosyl-L-homocysteine
-
Vaccinia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000005
-
brome mosaic virus RNA
-
Vaccinia virus
0.002
-
S-adenosyl-L-methionine
-
Vaccinia virus

Localization

Localization Comment Organism GeneOntology No. Textmining
viral nucleocapsid
-
Vaccinia virus 19013
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no divalent cations required Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + m7G(5')pppR-RNA Vaccinia virus final step in formation of 7-methylguanosine(5')pppNm-cap structure of viral RNA S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
?

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-
Vaccinia virus WR
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA] R may be guanine or adenosine, mechanism Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates are RNAs with pN-, ppN-, guanine(5')pppN-terminals Vaccinia virus ?
-
?
additional information no substrates are RNAs with pN-, ppN-, guanine(5')pppN-terminals Vaccinia virus WR ?
-
?
S-adenosyl-L-methionine + brome mosaic virus RNA
-
Vaccinia virus ?
-
r
S-adenosyl-L-methionine + m7G(5')pppR-RNA poor substrates are m7GpppN-dinucleotides Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA much less active with poly(G), poly(U) and poly(C) Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA unmethylated vaccinia virus mRNA Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA methylates 2'-O-position of penultimate nucleoside of RNA, no methylation of internal sites Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA best substrates are poly(A)-nucleosides and poly-(I)-nucleosides with m7G(5')pppN-ends Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA specific, in vitro methyl group acceptors are polyribonucleosides containing 7-methylguanine(5')pppG-terminals Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA brome mosaic virus RNA ending in m7G(5')pppG as substrate Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA final step in formation of 7-methylguanosine(5')pppNm-cap structure of viral RNA Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
?
S-adenosyl-L-methionine + m7G(5')pppR-RNA poor substrates are m7GpppN-dinucleotides Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA much less active with poly(G), poly(U) and poly(C) Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA unmethylated vaccinia virus mRNA Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA methylates 2'-O-position of penultimate nucleoside of RNA, no methylation of internal sites Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Vaccinia virus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
and below, stable in the presence of 10% v/v glycerol and 250 mg/l bovine serum albumin Vaccinia virus
59
-
rapid inactivation Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Vaccinia virus

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8.1 about half-maximal activity at pH 6.6 and 8.1 Vaccinia virus