General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Vaccinia virus |
glycerol, 10% v/v stabilizes | Vaccinia virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-methylguanosine(5')pppN-dinucleotides | weak, competitive | Vaccinia virus | |
additional information | no inhibition by poly(A), poly(U), poly(C) or poly(G) | Vaccinia virus | |
NaCl | above 0.05 M | Vaccinia virus | |
S-adenosyl-L-homocysteine | - |
Vaccinia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000005 | - |
brome mosaic virus RNA | - |
Vaccinia virus | |
0.002 | - |
S-adenosyl-L-methionine | - |
Vaccinia virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
viral nucleocapsid | - |
Vaccinia virus | 19013 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no divalent cations required | Vaccinia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + m7G(5')pppR-RNA | Vaccinia virus | final step in formation of 7-methylguanosine(5')pppNm-cap structure of viral RNA | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | - |
- |
- |
Vaccinia virus WR | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA] | R may be guanine or adenosine, mechanism | Vaccinia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrates are RNAs with pN-, ppN-, guanine(5')pppN-terminals | Vaccinia virus | ? | - |
? | |
additional information | no substrates are RNAs with pN-, ppN-, guanine(5')pppN-terminals | Vaccinia virus WR | ? | - |
? | |
S-adenosyl-L-methionine + brome mosaic virus RNA | - |
Vaccinia virus | ? | - |
r | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | poor substrates are m7GpppN-dinucleotides | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | much less active with poly(G), poly(U) and poly(C) | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | unmethylated vaccinia virus mRNA | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | methylates 2'-O-position of penultimate nucleoside of RNA, no methylation of internal sites | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | best substrates are poly(A)-nucleosides and poly-(I)-nucleosides with m7G(5')pppN-ends | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | specific, in vitro methyl group acceptors are polyribonucleosides containing 7-methylguanine(5')pppG-terminals | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | brome mosaic virus RNA ending in m7G(5')pppG as substrate | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | final step in formation of 7-methylguanosine(5')pppNm-cap structure of viral RNA | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | poor substrates are m7GpppN-dinucleotides | Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | much less active with poly(G), poly(U) and poly(C) | Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | unmethylated vaccinia virus mRNA | Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | methylates 2'-O-position of penultimate nucleoside of RNA, no methylation of internal sites | Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | mRNA containing a 2'-O-methylpurine cap, R may be guanine or adenosine, prereplicative viral product | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Vaccinia virus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
and below, stable in the presence of 10% v/v glycerol and 250 mg/l bovine serum albumin | Vaccinia virus |
59 | - |
rapid inactivation | Vaccinia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Vaccinia virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.1 | about half-maximal activity at pH 6.6 and 8.1 | Vaccinia virus |