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Literature summary for 2.1.1.53 extracted from

  • Stenzel, O.; Teuber, M.; Draeger, B.
    Putrescine N-methyltransferase in Solanum tuberosum L., a calystegine-forming plant (2006), Planta, 223, 200-212.
    View publication on PubMed
Search for more literature for 2.1.1.53

Application

Application Comment Organism
additional information chimera proteins obtained by fusion of fragments of Solanum tuberosum pmt2 and Hyoscyamus niger pmt are active as PMT Hyoscyamus niger
additional information no functional protein translated from Solanum tuberosum pmt2, chimera proteins obtained by fusion of fragments of Solanum tuberosum pmt2 and Hyoscyamus niger pmt are active as PMT, if the initial part of pmt2 is used, indicating that a mutation in the terminal part of the gene causes insolubility of the enzyme Solanum tuberosum

Cloned(Commentary)

Cloned (Comment) Organism
-
 Hyoscyamus niger
pmt1 and pmt2 cloned into pET-21d vector, PMT1 expressed in Escherichia coli Rosetta-gami (DE3), PMT2 expressed in Escherichia coli BL21 (DE3) and Rosetta-gami (DE3), pmt1 yields active enzymes, while pmt2 expression results in insoluble protein Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
1,6-diaminohexane weaker inhibitor Solanum tuberosum
1,7-Diaminoheptane weaker inhibitor Solanum tuberosum
cadaverine strong inhibitor Solanum tuberosum
n-butylamine strong inhibitor Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 putrescine PMT1 Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38180
-
-
 Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Hyoscyamus niger Q9XJ41
-
-
Solanum tuberosum Q70AR0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hyoscyamus niger
by Ni2+ chelate affinity chromatography Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
root very weak signal Solanum tuberosum
-
tuber transcript only in potato tuber eyes at sprouting start and in 1 and 3 mm long sprouts Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information inactive with n-butylamine, 1,6-diaminohexane and 1,7-diaminoheptane, the PMT enzyme of potato appears to be specific for putrescine, it does not accept other mono-, di- or tri-amines Solanum tuberosum ?
-
 ?
S-adenosyl-L-methionine + putrescine
-
 Hyoscyamus niger S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
S-adenosyl-L-methionine + putrescine
-
 Solanum tuberosum S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Synonyms

Synonyms Comment Organism
PMT
-
 Hyoscyamus niger
PMT1
-
 Solanum tuberosum
PMT2
-
 Solanum tuberosum