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Literature summary for 1.97.1.B1 extracted from

  • Yamazaki, C.; Kashiwa, S.; Horiuchi, A.; Kasahara, Y.; Yamamura, S.; Amachi, S.
    A novel dimethylsulfoxide reductase family of molybdenum enzyme, Idr, is involved in iodate respiration by Pseudomonas sp. SCT (2020), Environ. Microbiol., 22, 2196-2212 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene idrA, DNA and amino acid sequence determination and analysis, sequence comparisons, the idr genes (idrABP1P2) form an operon-like structure, phylogenetic analysis and tree Pseudomonas sp. SCT

Inhibitors

Inhibitors Comment Organism Structure
2-heptyl-4-hydroxyquinoline N-oxide HQNO, inhibits groth on iodate and iodate reduction completely Pseudomonas sp. SCT
antimycin A inhibits groth on iodate and iodate reduction completely Pseudomonas sp. SCT
potassium cyanide inhibits groth on iodate and iodate reduction completely Pseudomonas sp. SCT
rotenone inhibits groth on iodate and iodate reduction completely Pseudomonas sp. SCT

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pseudomonas sp. SCT
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum the enzyme sequence includes a motif associated with a [3Fe-4S] cluster (CX2CX3CX70S) and a molybdopterin guanine dinucleotide coordination motif Pseudomonas sp. SCT

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
iodate + reduced acceptor Pseudomonas sp. SCT
-
iodite + H2O + acceptor
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. SCT A0A391NTR7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information Pseudomonas strain SCT grows on 4 mM iodate as the electron acceptor, but it is not able to grow on a concentration above 5 mM iodate, comparative proteomic analysis, overview Pseudomonas sp. SCT
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.15
-
cell extract, cells are grown under iodate-respiring conditions, pH and temperature not specified in the publication Pseudomonas sp. SCT

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
iodate + reduced acceptor
-
Pseudomonas sp. SCT iodite + H2O + acceptor
-
?
iodate + reduced methyl viologen
-
Pseudomonas sp. SCT iodite + H2O + methyl viologen
-
?

Synonyms

Synonyms Comment Organism
aioA
-
Pseudomonas sp. SCT
Idr
-
Pseudomonas sp. SCT
IdrA
-
Pseudomonas sp. SCT
PSCT_04485
-
Pseudomonas sp. SCT

Cofactor

Cofactor Comment Organism Structure
molybdenum cofactor
-
Pseudomonas sp. SCT

Expression

Organism Comment Expression
Pseudomonas sp. SCT the idr genes (idrABP1P2) form an operon-like structure, and their transcription is upregulated under iodate-respiring conditions, expression analysis and comparative proteomic analysis of strain SCT grown on iodate and nitrate, detailed overview up

General Information

General Information Comment Organism
evolution the enzyme belongs to a dimethylsulfoxide reductase family of molybdenum enzymes, gene PSCT_04485 encoding IdrA (AioA) defines a distinct clade within the dimethylsulfoxide (DMSO) reductase family. The idr genes (idrABP1P2) form an operon-like structure Pseudomonas sp. SCT
metabolism Idr proteins and high affinity terminal oxidases (Cbb3 and Cyd), various H2O2 scavengers, and chlorite (ClO2 -) dismutase-like proteins are expressed specifically or abundantly under iodate-respiring conditions. Quantitative transcription analysis of the genes potentially involved in iodate respiration Pseudomonas sp. SCT
physiological function Pseudomonas sp. strain SCT is capable of using iodate (IO3-) as a terminal electron acceptor for anaerobic respiration. Periplasmic iodate reductase (Idr) is a possible key enzyme. Idr is a respiratory iodate reductase, and both O2 and H2O2 are formed as by-products of iodate respiration. Iodate-dependent H2O2 formation occurs biologically during iodate respiration Pseudomonas sp. SCT