Cloned (Comment) | Organism |
---|---|
gene idrA, DNA and amino acid sequence determination and analysis, sequence comparisons, the idr genes (idrABP1P2) form an operon-like structure, phylogenetic analysis and tree | Pseudomonas sp. SCT |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-heptyl-4-hydroxyquinoline N-oxide | HQNO, inhibits groth on iodate and iodate reduction completely | Pseudomonas sp. SCT | |
antimycin A | inhibits groth on iodate and iodate reduction completely | Pseudomonas sp. SCT | |
potassium cyanide | inhibits groth on iodate and iodate reduction completely | Pseudomonas sp. SCT | |
rotenone | inhibits groth on iodate and iodate reduction completely | Pseudomonas sp. SCT |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas sp. SCT | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | the enzyme sequence includes a motif associated with a [3Fe-4S] cluster (CX2CX3CX70S) and a molybdopterin guanine dinucleotide coordination motif | Pseudomonas sp. SCT |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
iodate + reduced acceptor | Pseudomonas sp. SCT | - |
iodite + H2O + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. SCT | A0A391NTR7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Pseudomonas strain SCT grows on 4 mM iodate as the electron acceptor, but it is not able to grow on a concentration above 5 mM iodate, comparative proteomic analysis, overview | Pseudomonas sp. SCT | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.15 | - |
cell extract, cells are grown under iodate-respiring conditions, pH and temperature not specified in the publication | Pseudomonas sp. SCT |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
iodate + reduced acceptor | - |
Pseudomonas sp. SCT | iodite + H2O + acceptor | - |
? | |
iodate + reduced methyl viologen | - |
Pseudomonas sp. SCT | iodite + H2O + methyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aioA | - |
Pseudomonas sp. SCT |
Idr | - |
Pseudomonas sp. SCT |
IdrA | - |
Pseudomonas sp. SCT |
PSCT_04485 | - |
Pseudomonas sp. SCT |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdenum cofactor | - |
Pseudomonas sp. SCT |
Organism | Comment | Expression |
---|---|---|
Pseudomonas sp. SCT | the idr genes (idrABP1P2) form an operon-like structure, and their transcription is upregulated under iodate-respiring conditions, expression analysis and comparative proteomic analysis of strain SCT grown on iodate and nitrate, detailed overview | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to a dimethylsulfoxide reductase family of molybdenum enzymes, gene PSCT_04485 encoding IdrA (AioA) defines a distinct clade within the dimethylsulfoxide (DMSO) reductase family. The idr genes (idrABP1P2) form an operon-like structure | Pseudomonas sp. SCT |
metabolism | Idr proteins and high affinity terminal oxidases (Cbb3 and Cyd), various H2O2 scavengers, and chlorite (ClO2 -) dismutase-like proteins are expressed specifically or abundantly under iodate-respiring conditions. Quantitative transcription analysis of the genes potentially involved in iodate respiration | Pseudomonas sp. SCT |
physiological function | Pseudomonas sp. strain SCT is capable of using iodate (IO3-) as a terminal electron acceptor for anaerobic respiration. Periplasmic iodate reductase (Idr) is a possible key enzyme. Idr is a respiratory iodate reductase, and both O2 and H2O2 are formed as by-products of iodate respiration. Iodate-dependent H2O2 formation occurs biologically during iodate respiration | Pseudomonas sp. SCT |