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Literature summary for 1.8.1.7 extracted from
- Purification and characterization of glutathione reductase (E.C. 1.8.1.7) from bovine filarial worms Setaria cervi (2013), J. Parasit. Dis., 37, 94-104.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | product inhibition | Setaria cervi |  |
| K+ | inhibitory in 0.1-1.0 M concentration range | Setaria cervi | |
| Na+ | inhibitory in 0.1-1.0 M concentration range | Setaria cervi | |
| NADP+ | product inhibition | Setaria cervi | |
| NH4+ | inhibitory in 0.1-1.0 M concentration range | Setaria cervi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
NADPH | crude enzyme, at pH 7.0 and 22°C | Setaria cervi | |
| 0.021 | - |
NADPH | purified enzyme, at pH 7.0 and 22°C | Setaria cervi | |
| 0.03 | - |
NADP+ | purified enzyme at pH 7.0 and 22°C | Setaria cervi | |
| 0.0465 | - |
glutathione disulfide | crude enzyme, at pH 7.0 and 22°C | Setaria cervi | |
| 0.048 | - |
NADP+ | crude enzyme, at pH 7.0 and 22°C | Setaria cervi | |
| 0.05 | - |
glutathione disulfide | purified enzyme, at pH 7.0 and 22°C | Setaria cervi | |
| 5.9 | - |
glutathione | purified enzyme, at pH 7.0 and 22°C | Setaria cervi | |
| 6.8 | - |
glutathione | crude enzyme, at pH 7.0 and 22°C | Setaria cervi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Setaria cervi |
| 110000 | - |
gel filtration | Setaria cervi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH + H+ | Setaria cervi | - |
glutathione + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Setaria cervi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, ultrafiltration, CM-Sepharose column chromatography, DEAE-Sepharose column chromatography, GSHSepharose affinity column chromatography, and ADP-Sepharose affinity chromatography | Setaria cervi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0362 | - |
purified enzyme, at pH 7.0 and 22°C | Setaria cervi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 1 month, no appreciable effect of freezing and thawing on enzyme activity | Setaria cervi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH + H+ | - |
Setaria cervi | glutathione + NADP+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 55000, SDS-PAGE | Setaria cervi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione: NADP+ oxidoreductase | - |
Setaria cervi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
4 | there is no appreciable effect of freezing and thawing on enzyme activity for approximately 1 month | Setaria cervi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | broad pH optimum centered around pH 7.0 | Setaria cervi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Setaria cervi | |
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