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Literature summary for 1.6.3.2 extracted from

  • Castillo-Villanueva, A.; Mendez, S.T.; Torres-Arroyo, A.; Reyes-Vivas, H.; Oria-Hernandez, J.
    Cloning, expression and characterization of recombinant, NADH oxidase from Giardia lamblia (2016), Protein J., 35, 24-33.
    View publication on PubMed
Search for more literature for 1.6.3.2

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by dithiothreitol Giardia duodenalis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Giardia duodenalis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 92% inhibition at 0.1 mg/ml Giardia duodenalis
Hg2+ complete inhibition at 0.1 mg/ml Giardia duodenalis
p-mercuribenzoate 99% inhibition at 0.1 mg/ml Giardia duodenalis
Quinacrine 51% inhibition at 0.1 mg/ml Giardia duodenalis
Zn2+ 89% inhibition at 0.1 mg/ml Giardia duodenalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 NADH at pH 7.8 and 25°C Giardia duodenalis
0.0874
-
 NADPH at pH 7.8 and 25°C Giardia duodenalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
214000
-
 gel filtration Giardia duodenalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + O2 Giardia duodenalis
-
 NAD+ + H2O
-
 ?
NADPH + H+ + O2 Giardia duodenalis
-
 NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia duodenalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Giardia duodenalis

Storage Stability

Storage Stability Organism
-70°C, 50% (v/v) glycerol, at least 2 months, no loss of activity Giardia duodenalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + O2
-
 Giardia duodenalis NAD+ + H2O
-
 ?
NADPH + H+ + O2
-
 Giardia duodenalis NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 50300, calculated from amino acid sequence Giardia duodenalis
homotetramer 4 * 50000, SDS-PAGE Giardia duodenalis

Synonyms

Synonyms Comment Organism
NOX
-
 Giardia duodenalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Giardia duodenalis

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains 0.91 mol FAD per mol of monomer Giardia duodenalis