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Literature summary for 1.6.3.2 extracted from

  • Nisar, M.A.; Rashid, N.; Bashir, Q.; Gardner, Q.T.; Shafiq, M.H.; Akhtar, M.
    TK1299, a highly thermostable NAD(P)H oxidase from Thermococcus kodakaraensis exhibiting higher enzymatic activity with NADPH (2013), J. Biosci. Bioeng., 116, 39-44.
    View publication on PubMed
Search for more literature for 1.6.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 NADH pH 7.8, 75°C Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48610
-
 6 * 48610, sequence calculation Thermococcus kodakarensis
49375
-
 6 * 49375, mass spectrometry Thermococcus kodakarensis
300000
-
 gel filtration Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JGP4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 NADH + 3 H+ + 2 O2 approximately 75% H2O and 25% H2O is produced. Km value of the enzyme towards NADH and NADPH is almost the same whereas specific activity is higher with NADPH compared to NADH Thermococcus kodakarensis 3 NAD+ + H2O
-
 ?
3 NADPH + 3 H+ + 2 O2 approximately 87% H2O and 13% H2O2 is produced. Km value of the enzyme towards NADH and NADPH is almost the same whereas specific activity is higher with NADPH compared to NADH Thermococcus kodakarensis 3 NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 49375, mass spectrometry Thermococcus kodakarensis
hexamer 6 * 48610, sequence calculation Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
KOD1
-
 Thermococcus kodakarensis
NAD(P)H oxidase
-
 Thermococcus kodakarensis
TK1299
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 80 70°C: about 60% of maximal activity, 80°C: about 70% of maximal activity Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 150 min, less than 5% loss of activity Thermococcus kodakarensis
120
-
 secondary structure of the enzyme is not stable at this temperature Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
68
-
 NADH pH 7.8, 75°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermococcus kodakarensis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 pH 7.5: about 70% of maximal activity, pH 8.5: about 70% of maximal activity Thermococcus kodakarensis

Cofactor

Cofactor Comment Organism Structure
FAD tightly bound, 0.5 FAD attached per subunit Thermococcus kodakarensis