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Literature summary for 1.6.3.2 extracted from

  • Brown, D.M.; Upcroft, J.A.; Upcroft, P.
    A H2O-producing NADH oxidase from the protozoan parasite Giardia duodenalis (1996), Eur. J. Biochem., 241, 155-161.
    View publication on PubMed
Search for more literature for 1.6.3.2

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 0.2 mM, 97% inhibition Giardia duodenalis
H2O2 0.5 mM, 50% inhibition Giardia duodenalis
HgCl2 0.5 mM, 80% inhibition Giardia duodenalis
additional information no inhibition by EDTA Giardia duodenalis
p-chloromercuribenzoate 0.05 mM, complete inhibition Giardia duodenalis
Quinacrine 1 mM, 73% inhibition Giardia duodenalis
Quinine 0.2 mM, 50% inhibition Giardia duodenalis
ZnSO4 0.1 mM, 98% inhibition Giardia duodenalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
 NADH pH 7.8, 22°C Giardia duodenalis
0.016
-
 NADPH pH 7.8, 22°C Giardia duodenalis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity is not enhanced by the addition of various heavy metals which suggests that the enzyme does not contain any metal ions as co-factors Giardia duodenalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45116
-
 1 * 45116, calculated from sequence Giardia duodenalis
46000
-
 1 * 46000, SDS-PAGE Giardia duodenalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 NADPH + 2 H+ + O2 Giardia duodenalis the enzyme may be involved in maintenance of an optimum intracellular redox ratio 2 NADP+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 Giardia duodenalis BRIS/83/HEPU/106 the enzyme may be involved in maintenance of an optimum intracellular redox ratio 2 NADP+ + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia duodenalis Q7M4I9
-
-
Giardia duodenalis BRIS/83/HEPU/106 Q7M4I9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Giardia duodenalis

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Giardia duodenalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 NADH + H+ + O2 NADPH is more efficient as electron donor compared to NADH Giardia duodenalis NAD+ + 2 H2O
-
 ?
2 NADH + H+ + O2 NADPH is more efficient as electron donor compared to NADH Giardia duodenalis BRIS/83/HEPU/106 NAD+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia duodenalis 2 NADP+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically Giardia duodenalis 2 NADP+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia duodenalis BRIS/83/HEPU/106 2 NADP+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically Giardia duodenalis BRIS/83/HEPU/106 2 NADP+ + 2 H2O
-
 ?
additional information cytochrome c is not reduced under any assay conditions used Giardia duodenalis ?
-
 ?
additional information cytochrome c is not reduced under any assay conditions used Giardia duodenalis BRIS/83/HEPU/106 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Giardia duodenalis
monomer 1 * 45116, calculated from sequence Giardia duodenalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Giardia duodenalis

pH Range

pH Minimum pH Maximum Comment Organism
7 9 active over the range pH 7-9 Giardia duodenalis

Cofactor

Cofactor Comment Organism Structure
FAD FAD is noncoveltly bound, 1 mol of FAD per mol of polypeptide Giardia duodenalis
NADH NADPH is more efficient as electron donor compared to NADH Giardia duodenalis
NADPH NADPH is more efficient as electron donor compared to NADH Giardia duodenalis

General Information

General Information Comment Organism
physiological function the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia duodenalis