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Literature summary for 1.5.1.8 extracted from

  • Schmidt, D.; Rizzi, V.; Gaziola, S.A.; Medici, L.O.; Vincze, E.; Kozak, M.; Lea, P.J.; Azevedo, R.A.
    Lysine metabolism in antisense C-hordein barley grains (2015), Plant Physiol. Biochem., 87, 73-83 .
    View publication on PubMed
Search for more literature for 1.5.1.8

Activating Compound

Activating Compound Comment Organism Structure
additional information C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines, overview Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
additional information C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines., overview Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH + H+ Hordeum vulgare
-
 N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
 cv. Golden Promise
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially through ammonium sulfate fractionation and desalting gel filtration Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
seed grain, spikes, at different developmental stages: early milk, late milk, and soft dough. The enzyme exhibits the highest activity at the late milk stage Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00039
-
 enzyme from grain at late milk stage, pH 7.4, 30°C Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH + H+
-
 Hordeum vulgare N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
LKR
-
 Hordeum vulgare
lysine alpha-ketoglutarate reductase
-
 Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Hordeum vulgare

Expression

Organism Comment Expression
Hordeum vulgare C-hordein suppression alters the enzyme activity differentially in different developmental stages, the extent varies among the transgenic lines, overview additional information

General Information

General Information Comment Organism
metabolism the enzyme is involved in L-lysine degradation Hordeum vulgare