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Literature summary for 1.5.1.53 extracted from

  • Zhou, J.; Kang, S.S.; Wong, P.W.K.; Fournier, B.; Rozen, R.
    Purification and characterization of methylenetetrahydrofolate reductase from human cadaver liver [published erratum appears in Biochem Med Metab Biol 1990 Oct;44(2):200] (1990), Biochem. Med. Metab. Biol., 43, 234-242.
    View publication on PubMed
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Application

Application Comment Organism
medicine patients with severe methylenetetrahydrofolate reductase deficiency show neurologic disorders, mental retardation, and rapid deterioration Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-methyltetrahydrofolate
-
 Homo sapiens
dihydrofolate
-
 Homo sapiens
additional information not inhibitory: folic acid, S-adenosyl-L-methionine Homo sapiens
tetrahydrofolate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 NADPH pH 6.7, 25°C Homo sapiens
0.038
-
 5-methyltetrahydrofolate pH not specified in the publication, temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH + H+ Homo sapiens
-
 5-methyltetrahydrofolate + NADP+
-
 ?
5-methyltetrahydrofolate + oxidized menadione Homo sapiens
-
 5,10-methylenetetrahydrofolate + reduced menadione
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.367
-
 substrate 5-methyltetrahydrofolate, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH + H+
-
 Homo sapiens 5-methyltetrahydrofolate + NADP+
-
 ?
5-methyltetrahydrofolate + oxidized menadione
-
 Homo sapiens 5,10-methylenetetrahydrofolate + reduced menadione
-
 ?
NADPH + H+ + oxidized menadione
-
 Homo sapiens NADP+ + reduced menadione
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 substrate 5,10-methylenetetrahydrofolate Homo sapiens
6.6
-
 substrate 5-methyltetrahydrofolate Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD 32% residual activity in absence of FAD Homo sapiens