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Literature summary for 1.5.1.48 extracted from

  • Mitsukura, K.; Suzuki, M.; Shinoda, S.; Kuramoto, T.; Yoshida, T.; Nagasawa, T.
    Purification and characterization of a novel (R)-imine reductase from Streptomyces sp. GF3587 (2011), Biosci. Biotechnol. Biochem., 75, 1778-1782.
    View publication on PubMed
Search for more literature for 1.5.1.48

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 78% inhibition Streptomyces sp.
2,2'-bipyridyl 1 mM, 75% inhibition Streptomyces sp.
4-chloromercuribenzoate 0.1 mM, 97% inhibition Streptomyces sp.
8-hydroxyquinoline 1 mM, 94% inhibition Streptomyces sp.
AgNO3 1 mM, 97% inhibition Streptomyces sp.
CuCl2 1 mM, 41% inhibition Streptomyces sp.
Cuprizone 0.1 mM, 87% inhibition Streptomyces sp.
HgCl2 1 mM, 75% inhibition Streptomyces sp.
additional information no inhibition: EDTA, Tiron, CaCl2, CdCl2, CoCl2, FeCl3, LiOH, MgCl2, NiCl2, ZnSO4, semicarbazide, diethylamine cysteamine, 3-(aminomethyl)pyridine, iodoacetate, (R)-(+)-cycloserine, diethyldithiocarbamiate, N-ethylmaleimide Streptomyces sp.
PMSF 0.1 mM, 93% inhibition Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 NADPH 30°C, pH 7.0 Streptomyces sp.
3.52
-
 2-methyl-1-pyrroline 30°C, pH 7.0 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Streptomyces sp.
65000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl-1-pyrroline + NADPH + H+ Streptomyces sp.
-
 (R)-2-methylpyrrolidine + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00032
-
 30°C, pH 7.0, oxidation of 2-methyl-1-pyrroline Streptomyces sp.
10.2
-
 30°C, pH 7.0, reduction of 2-methyl-1-pyrroline Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-1-pyrroline + NADPH + H+
-
 Streptomyces sp. (R)-2-methylpyrrolidine + NADP+
-
 r
2-methyl-1-pyrroline + NADPH + H+ the enzyme is very specific for 2-methyl-1-pyrroline as substrate. The purified enzyme catalyzes the formation of (R)-2-methylpyrrolidine with 99% enantiomeric excess. The enzyme shows also oxidation activity for (R)-2-methyl-1-pyrroline under alkaline pH (10-11.5) conditions. No activity with (S)-2-methylpyrrolidine. No activity with NADH Streptomyces sp. (R)-2-methylpyrrolidine + NADP+
-
 r
additional information no activity with DELTA1-pyrroline-2-carboxylate, methyl-DELTA1-pyrroline-2-carboxylate, dihydrofolate, DELTA1-piperidine-2-carboxylate, 1-methyl-3,4-dihydroisoquinoline, 1-methyl-6,7-dimethoxy-3,4-dihydroisoquinoline, N-benzylidenebenzylamine, (E)-N-(1-phenylethylidene)benzeneamine ketone, 4-hydroxy-2-butanone, ethyl-3-oxobutanoate, ethyl 4-chloro-3-oxobutanoate, 3-2,2,6-trimethyl-2-cyclohexen-1,4-dione, 2-methylfuran, 2-methylimidazole, 2-methylthiazole, 2-methylpyrimidine, 2-methylpyrazine, 1,4,5,6-tetrahydropyrimidine Streptomyces sp. ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 32000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
(R)-imine reductase
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces sp.
50
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable below, 30 min Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 reduction of 2-methyl-1-pyrroline Streptomyces sp.
7
-
 assay at Streptomyces sp.
11
-
 oxidation of (R)-2-methylpyrrolidine Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.7 pH 6.2: about 35% of maximal activity, pH 8.7: about 55% of maximal activity, reduction of 2-methyl-1-pyrroline Streptomyces sp.
10.2 11.7 pH 6.2: about 60% of maximal activity, pH 8.7: about 60% of maximal activity, oxidation of (R)-2-methylpyrrolidine Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
NADPH NADPH-dependent enzyme. No activity with NADH Streptomyces sp.