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Literature summary for 1.4.99.6 extracted from
- Efficient synthesis of D-branched-chain amino acids and their labeled compounds with stable isotopes using D-amino acid dehydrogenase (2014), Appl. Microbiol. Biotechnol., 98, 1135-1143.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells | Ureibacillus thermosphaericus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32.3 | - |
2-oxo-4-methylvaleric acid | at pH 10.5 and 50°C | Ureibacillus thermosphaericus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ureibacillus thermosphaericus | - |
- |
- |
| Ureibacillus thermosphaericus A1 (NBRC 108682) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose column chromatography, and gel filtration | Ureibacillus thermosphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus | D-isoleucine + H2O + NADP+ | yield of 45% after incubation for 1 h at 50 °C | ? | |
| (3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus A1 (NBRC 108682) | D-isoleucine + H2O + NADP+ | yield of 45% after incubation for 1 h at 50 °C | ? | |
| 2-oxo-3-methylbutyric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus | D-valine + H2O + NADP+ | yield of 83% after incubation for 1 h at 50 °C | ? | |
| 2-oxo-3-methylbutyric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus A1 (NBRC 108682) | D-valine + H2O + NADP+ | yield of 83% after incubation for 1 h at 50 °C | ? | |
| 2-oxo-4-methylvaleric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus | D-leucine + H2O + NADP+ | yield of 91% after incubation for 1 h at 50 °C | ? | |
| 2-oxo-4-methylvaleric acid + NH3 + NADPH + H+ | - |
Ureibacillus thermosphaericus A1 (NBRC 108682) | D-leucine + H2O + NADP+ | yield of 91% after incubation for 1 h at 50 °C | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-amino acid dehydrogenase | - |
Ureibacillus thermosphaericus |
| DAADH | - |
Ureibacillus thermosphaericus |
| NADP+-dependent D-amino acid dehydrogenase | - |
Ureibacillus thermosphaericus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
temperature optimum for D-leucine synthesis | Ureibacillus thermosphaericus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5700 | - |
2-oxo-4-methylvaleric acid | at pH 10.5 and 50°C | Ureibacillus thermosphaericus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
pH-optimum for D-leucine synthesisin glycine-KOH buffer | Ureibacillus thermosphaericus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | the yield is highest in the presence of 0.5 mM NADP+ | Ureibacillus thermosphaericus | |
| NADPH | dependent on | Ureibacillus thermosphaericus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 180 | - |
2-oxo-4-methylvaleric acid | at pH 10.5 and 50°C | Ureibacillus thermosphaericus | |
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Release 2023.1 (January 2023)
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