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Literature summary for 1.4.99.6 extracted from
- D-Amino acid dehydrogenase (Pseudomonas fluorescens) (1971), Methods Enzymol., 17B, 623-624.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| very unstable | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | inhibition of the dichlorophenolindophenol-specific enzyme at high concentrations | Pseudomonas fluorescens |  |
| D-Amino acids | various D-amino acids competitively inhibit D-kynurenine deamination | Pseudomonas fluorescens | |
| p-chloromercuribenzoic acid | 90% inhibition of the methylene blue specific, 70% inhibition of the dichlorophenolindophenol specific enzyme at 0.5 mM | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
methylene blue | specific acceptor, cosubstrate: D-kynurenine | Pseudomonas fluorescens | |
| 0.07 | - |
2,6-dichlorophenolindophenol | specific acceptor, cosubstrate: D-kynurenine | Pseudomonas fluorescens | |
| 0.45 | - |
D-kynurenine | both methylene blue and 2,6-dichlorophenolindophenol specific enzymes | Pseudomonas fluorescens | |
| 0.47 | - |
D-tryptophan | both methylene blue and 2,6-dichlorophenolindophenol specific enzymes | Pseudomonas fluorescens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| near homogeneity, two enzymes with different acceptor specificities, fractionation and chromatography techniques | Pseudomonas fluorescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | one methylene blue specific, one 2,6-dichlorophenolindophenol specific enzyme, the methylene blue specific enzyme is only detectable in D-tryptophan grown cells | Pseudomonas fluorescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.113 | - |
methylene blue specific enzyme | Pseudomonas fluorescens |
| 0.213 | - |
2,6-dichlorophenolindophenol specific enzyme | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + 2,6-dichlorophenolindophenol | low activity | Pseudomonas fluorescens | pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-alanine + H2O + methylene blue | low activity | Pseudomonas fluorescens | pyruvate + NH3 + reduced methylene blue | - |
? | |
| D-asparagine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-asparagine + H2O + methylene blue | - |
Pseudomonas fluorescens | 2-oxosuccinamic acid + NH3 + reduced methylene blue | - |
? | |
| D-cysteine + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-mercapto-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
| D-cystine + H2O + methylene blue | - |
Pseudomonas fluorescens | ? + NH3 + reduced methylene blue | - |
? | |
| D-histidine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-histidine + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
| D-isoleucine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-isoleucine + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-methyl-2-oxopentanoate + NH3 + reduced methylene blue | - |
? | |
| D-kynurenine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-kynurenine + H2O + methylene blue | - |
Pseudomonas fluorescens | 4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced methylene blue | - |
? | |
| D-leucine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-leucine + H2O + methylene blue | - |
Pseudomonas fluorescens | 4-methyl-2-oxopentanoate + NH3 + reduced methylene blue | - |
? | |
| D-methionine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-norleucine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 2-oxohexanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-norleucine + H2O + methylene blue | - |
Pseudomonas fluorescens | 2-oxohexanoate + NH3 + reduced methylene blue | - |
? | |
| D-norvaline + H2O + methylene blue | - |
Pseudomonas fluorescens | 2-oxopentanoate + NH3 + reduced methylene blue | - |
? | |
| D-phenylalanine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-phenylalanine + H2O + methylene blue | - |
Pseudomonas fluorescens | phenylpyruvate + NH3 + reduced methylene blue | - |
? | |
| D-serine + H2O + 2,6-dichlorophenolindophenol | low activity | Pseudomonas fluorescens | 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-serine + H2O + methylene blue | low activity | Pseudomonas fluorescens | 3-hydroxy-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
| D-threonine + H2O + 2,6-dichlorophenolindophenol | low activity | Pseudomonas fluorescens | 3-hydroxy-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-threonine + H2O + methylene blue | low activity | Pseudomonas fluorescens | 3-hydroxy-2-oxobutanoate + NH3 + reduced methylene blue | - |
? | |
| D-tryptophan + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 3-indole-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-tryptophan + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-indole-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
| D-tyrosine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-tyrosine + H2O + methylene blue | - |
Pseudomonas fluorescens | 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced methylene blue | - |
? | |
| D-valine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-valine + H2O + methylene blue | - |
Pseudomonas fluorescens | 2-oxoisopentanoate + NH3 + reduced methylene blue | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | both methylene blue and 2,6-dichlorophenolindophenol specific enzymes | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas fluorescens | |
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Release 2023.1 (January 2023)
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