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Literature summary for 1.4.3.20 extracted from

  • Sehanobish, E.; Shin, S.; Sanchez-Amat, A.; Davidson, V.L.
    Steady-state kinetic mechanism of LodA, a novel cysteine tryptophylquinone-dependent oxidase (2014), FEBS Lett., 588, 752-756 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Marinomonas mediterranea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
 L-lysine at pH 7.5 and 25°C Marinomonas mediterranea
0.0372
-
 O2 at pH 7.5 and 25°C Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Marinomonas mediterranea
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O Marinomonas mediterranea NBRC 103028
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Marinomonas mediterranea F2JXJ3
-
-
Marinomonas mediterranea NBRC 103028 F2JXJ3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and DEAE cellulose column chromatography Marinomonas mediterranea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2 + H2O
-
 Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O
-
 Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
additional information alternative electron acceptors like 2,6-dichloroinophenol, ferricyanide, NAD+, amicyanin or cytochrome C-550 cannot effectively substitute for O2 in the reaction Marinomonas mediterranea ?
-
 ?
additional information alternative electron acceptors like 2,6-dichloroinophenol, ferricyanide, NAD+, amicyanin or cytochrome C-550 cannot effectively substitute for O2 in the reaction Marinomonas mediterranea NBRC 103028 ?
-
 ?

Synonyms

Synonyms Comment Organism
LodA
-
 Marinomonas mediterranea
lysine-epsilon-oxidase
-
 Marinomonas mediterranea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 L-lysine at pH 7.5 and 25°C Marinomonas mediterranea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Marinomonas mediterranea

Cofactor

Cofactor Comment Organism Structure
cysteine tryptophylquinone
-
 Marinomonas mediterranea