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Literature summary for 1.4.3.20 extracted from

  • Chacon-Verdu, M.D.; Campillo-Brocal, J.C.; Lucas-Elio, P.; Davidson, V.L.; Sanchez-Amat, A.
    Characterization of recombinant biosynthetic precursors of the cysteine tryptophylquinone cofactors of l-lysine-epsilon-oxidase and glycine oxidase from Marinomonas mediterranea (2015), Biochim. Biophys. Acta, 1854, 1123-1131 .
    View publication on PubMed
Search for more literature for 1.4.3.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Marinomonas mediterranea

Protein Variants

Protein Variants Comment Organism
W581A inactive Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Marinomonas mediterranea
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O Marinomonas mediterranea NBRC 103028
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Marinomonas mediterranea F2JXJ3
-
-
Marinomonas mediterranea NBRC 103028 F2JXJ3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Marinomonas mediterranea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2 + H2O
-
 Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O
-
 Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 85000, SDS-PAGE Marinomonas mediterranea
? x * 83071, calculated from amino acid sequence Marinomonas mediterranea

Synonyms

Synonyms Comment Organism
L-lysine-epsilon-oxidase
-
 Marinomonas mediterranea
LodA
-
 Marinomonas mediterranea

Cofactor

Cofactor Comment Organism Structure
cysteine tryptophylquinone
-
 Marinomonas mediterranea