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Literature summary for 1.3.99.30 extracted from
- A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa (2000), Fungal Genet. Biol., 30, 147-153.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | more than 2fold stimulation of activity compared to control, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa | Neurospora crassa |  |
| NADP+ | stimulation is lower than with NAD+, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa | Neurospora crassa | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Neurospora crassa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, SDS-PAGE | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 15-cis-phytoene + 5 acceptor | Neurospora crassa | the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor | overall reaction | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxylycopene + acceptor | - |
Neurospora crassa | 1-hydroxy-3',4'-didehydrolycopene + reduced acceptor | - |
? | |
| 1-hydroxyneurosporene + acceptor | - |
Neurospora crassa | 1-hydroxylycopene + reduced acceptor | - |
? | |
| 15-cis-phytoene + 5 acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
| 15-cis-phytoene + 5 acceptor | the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene | Neurospora crassa | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
| all-trans-lycopene + acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + reduced acceptor | - |
? | |
| all-trans-neurosporene + 2 acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + 2 reduced acceptor | - |
? | |
| all-trans-phytofluene + 4 acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + 4 reduced acceptor | - |
? | |
| all-trans-zeta-carotene + 3 acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + 3 reduced acceptor | - |
? | |
| additional information | gamma-carotene and 1,1'-dihydroxylycopene are not accepted as substrate | Neurospora crassa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Neurospora crassa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Al-1 | - |
Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of FAD results in product formation close to that of the control without additions | Neurospora crassa |
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