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Literature summary for 1.3.1.38 extracted from
- Studies on the metabolism of unsaturated fatty acids. XIV. Purification and properties of NADPH-dependent trans-2-enoyl-CoA reductase of Escherichia coli K-12 (1984), J. Biochem., 95, 1315-1321.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no flavoprotein | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | i.e. DTNB, inhibition can be prevented by preincubation with NADPH, thus a SH-group is involved in cofactor binding | Escherichia coli |  |
| bovine serum albumin | - |
Escherichia coli | |
| decanoyl-CoA | - |
Escherichia coli | |
| lauroyl-CoA | - |
Escherichia coli | |
| additional information | not inhibitory: methylglyoxal | Escherichia coli | |
| myristoyl-CoA | - |
Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| NADP+ | - |
Escherichia coli | |
| octanoyl-CoA | - |
Escherichia coli | |
| p-hydroxymercuribenzoate | - |
Escherichia coli | |
| palmitoleoyl-CoA | - |
Escherichia coli | |
| palmitoyl-CoA | - |
Escherichia coli | |
| Saturated CoA analogs | - |
Escherichia coli | |
| Triton X-100 | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0037 | - |
trans-2-dodecenoyl-CoA | - |
Escherichia coli | |
| 0.0049 | - |
NADPH | - |
Escherichia coli | |
| 0.0071 | - |
trans-2-decenoyl-CoA | - |
Escherichia coli | |
| 0.011 | - |
trans-2-octenoyl-CoA | - |
Escherichia coli | |
| 0.048 | - |
trans-2-hexenoyl-CoA | - |
Escherichia coli | |
| 0.072 | - |
trans-2-crotonyl-CoA | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Escherichia coli |
| 80000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-2-hexenoyl-CoA + NADPH + H+ | Escherichia coli | - |
hexanoyl-CoA + NADP+ | - |
? | |
| trans-2-octenoyl-CoA + NADPH + H+ | Escherichia coli | - |
octanoyl-CoA + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
purified enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, Tris-HCl buffer, pH 8.0, 10% v/v glycerol | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotonyl-CoA + NADPH | - |
Escherichia coli | butanoyl-CoA + NADP+ | - |
? | |
| additional information | activity decreases with increasing chain length of substrates | Escherichia coli | ? | - |
? | |
| trans-2-decenoyl-CoA + NADPH | - |
Escherichia coli | decanoyl-CoA + NADP+ | - |
? | |
| trans-2-dodecenoyl-CoA | - |
Escherichia coli | ? | - |
r | |
| trans-2-hexenoyl-CoA + NADPH | - |
Escherichia coli | hexanoyl-CoA + NADP+ | - |
? | |
| trans-2-hexenoyl-CoA + NADPH + H+ | - |
Escherichia coli | hexanoyl-CoA + NADP+ | - |
? | |
| trans-2-octenoyl-CoA + NADPH | - |
Escherichia coli | octanoyl-CoA + NADP+ | - |
? | |
| trans-2-octenoyl-CoA + NADPH + H+ | - |
Escherichia coli | octanoyl-CoA + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | specific for | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00054 | - |
palmitoleoyl-CoA | - |
Escherichia coli | |
| 0.0025 | - |
palmitoyl-CoA | - |
Escherichia coli | |
| 0.0092 | - |
myristoyl-CoA | - |
Escherichia coli | |
| 0.045 | - |
lauroyl-CoA | - |
Escherichia coli | |
| 0.063 | - |
decanoyl-CoA | - |
Escherichia coli | |
| 0.198 | - |
octanoyl-CoA | - |
Escherichia coli | |
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