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Literature summary for 1.2.1.28 extracted from

  • Chalmers, R.M.; Scott, A.J.; Fewson, C.A.
    Purification of the benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by the TOL plasmid pWW53 of Pseudomonas putida MT53 and their preliminary comparison with benzyl alcohol dehydrogenase and benzaldehyde dehydrogenases I and II from Acinetobacter calcoaceticus (1990), J. Gen. Microbiol., 136, 637-643.
No PubMed abstract available
Search for more literature for 1.2.1.28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00038
-
 3-methylbenzaldehyde
-
 Pseudomonas putida
0.00097
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
0.0012
-
 3-methylbenzaldehyde
-
 Acinetobacter calcoaceticus
0.003
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
0.003
-
 4-methylbenzaldehyde
-
 Acinetobacter calcoaceticus
0.026
-
 2-Methylbenzaldehyde
-
 Pseudomonas putida
0.088
-
 2-Methylbenzaldehyde
-
 Acinetobacter calcoaceticus
0.33
-
 NAD+
-
 Pseudomonas putida
0.46
-
 benzaldehyde
-
 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
K+ or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ Acinetobacter calcoaceticus
K+ or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56300
-
 4 * 56300, SDS-PAGE Pseudomonas putida
122000 126000 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + NAD+ + H2O Pseudomonas putida
-
 benzoate + NADH + H+
-
 ir
benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
 benzoate + NADH + H+
-
 ir

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.2
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 2-methylbenzoate + NADH
-
 ?
2-methylbenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 2-methylbenzoate + NADH
-
 ?
3-methylbenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 3-methylbenzoate + NADH
-
 ?
3-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methylbenzoate + NADH
-
 ?
4-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 4-methylbenzoate + NADH
-
 ?
4-methylbenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 4-methylbenzoate + NADH
-
 ?
benzaldehyde + NAD+ + H2O also active with NADP+, see also EC 1.2.1.7 Pseudomonas putida benzoate + NADH + H+
-
 ir
benzaldehyde + NAD+ + H2O also active with NADP+, see also EC 1.2.1.7 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ir
benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida benzoate + NADH + H+
-
 ir
benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ir
benzaldehyde + NAD+ + H2O also active with NADP+, see also EC 1.2.1.7 Pseudomonas putida MT53 benzoate + NADH + H+
-
 ir

Subunits

Subunits Comment Organism
tetramer
-
 Acinetobacter calcoaceticus
tetramer 4 * 56300, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas putida
NAD+
-
 Acinetobacter calcoaceticus
NADP+
-
 Acinetobacter calcoaceticus
NADP+ enzyme on Pseudomonas plasmid Pseudomonas putida