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Literature summary for extracted from

  • Shimomura, O.; Johnson, F.H.; Kohama, Y.
    Reactions involved in bioluminescence systems of limpet (Latia neritoides) and luminous bacteria (1972), Proc. Natl. Acad. Sci. USA, 69, 2086-2089.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol strong increase of activity Latia neritoides
Purple protein requirement Latia neritoides


Organism UniProt Comment Textmining
Latia neritoides
freshwater limpet

Purification (Commentary)

Purification (Comment) Organism
Latia neritoides


Reaction Comment Organism Reaction ID
Latia luciferin + reduced acceptor + 2 O2 = oxidized Latia luciferin + CO2 + formate + acceptor + H2O + hnu possibly two enzyme components are involved in the reaction, an oxygenase followed by a monooxygenase for the actual light emitting step Latia neritoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-methyl-4-(2,6,6-trimethyl-1-cyclohex-1-yl)-1-buten-1-ol-formate + NADH + O2 trivial name Latia luciferin Latia neritoides oxidized Latia luciferin + NAD+ + CO2 + H2O + formate + light

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
assay at Latia neritoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
assay at Latia neritoides


Cofactor Comment Organism Structure
ascorbate activation, especially together with optimal NADH Latia neritoides
FMN flavoprotein, reductase component Latia neritoides
FMNH2 luciferase Latia neritoides
NADH reductase component Latia neritoides