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Literature summary for 1.14.99.21 extracted from

  • Shimomura, O.; Johnson, F.H.; Kohama, Y.
    Reactions involved in bioluminescence systems of limpet (Latia neritoides) and luminous bacteria (1972), Proc. Natl. Acad. Sci. USA, 69, 2086-2089.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.21

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol strong increase of activity Latia neritoides
Purple protein requirement Latia neritoides

Organism

Organism UniProt Comment Textmining
Latia neritoides
-
 freshwater limpet
-

Purification (Commentary)

Purification (Comment) Organism
-
 Latia neritoides

Reaction

Reaction Comment Organism Reaction ID
Latia luciferin + reduced acceptor + 2 O2 = oxidized Latia luciferin + CO2 + formate + acceptor + H2O + hnu possibly two enzyme components are involved in the reaction, an oxygenase followed by a monooxygenase for the actual light emitting step Latia neritoides
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-methyl-4-(2,6,6-trimethyl-1-cyclohex-1-yl)-1-buten-1-ol-formate + NADH + O2 trivial name Latia luciferin Latia neritoides oxidized Latia luciferin + NAD+ + CO2 + H2O + formate + light
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10
-
 assay at Latia neritoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Latia neritoides

Cofactor

Cofactor Comment Organism Structure
ascorbate activation, especially together with optimal NADH Latia neritoides
FMN flavoprotein, reductase component Latia neritoides
FMNH2 luciferase Latia neritoides
NADH reductase component Latia neritoides