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Literature summary for 1.14.19.2 extracted from

  • Tupec, M.; Culka, M.; Machara, A.; Machacek, S.; Bim, D.; Svatos, A.; Rulisek, L.; Pichova, I.
    Understanding desaturation/hydroxylation activity of castor stearoyl DELTA9-desaturase through rational mutagenesis (2022), Comput. Struct. Biotechnol. J., 20, 1378-1388.
    View publication on PubMed
Search for more literature for 1.14.19.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Lemo21(DE3) cells Ricinus communis

Protein Variants

Protein Variants Comment Organism
D101I/H203I the mutation results in an approximately 2fold decrease in desaturase activity with stearoyl-CoA substrate compared to the wild type enzyme Ricinus communis
D101I/H203I/T206V/C222F the mutation results in an approximately 9fold decrease in desaturase activity with stearoyl-CoA substrate compared to the wild type enzyme Ricinus communis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Ricinus communis
-
 oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-hydroxystearoyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Ricinus communis (9Z)-11-hydroxyoctadec-9-enoyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
additional information when presented with 9-fluorinated acyl-ACPs, the enzyme hydroxylates at C10 and C11, resulting in vicinal or methylene-bridged fluorohydrins Ricinus communis ?
-
 ?
oleoyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Ricinus communis erythro-9,10-dihydroxystearoyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
stearoyl-CoA + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Ricinus communis oleoyl-CoA + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Ricinus communis oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
DELTA9D
-
 Ricinus communis
stearoyl DELTA9-desaturase
-
 Ricinus communis

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Ricinus communis
NADPH
-
 Ricinus communis