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Literature summary for 1.14.13.196 extracted from

  • Romero, E.; Fedkenheuer, M.; Chocklett, S,W.; Qi, J.; Oppenheimer, M.; Sobrado, P.
    Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase (2012), Biochim. Biophys. Acta, 1824, 850-857.
    View publication on PubMed
Search for more literature for 1.14.13.196

Application

Application Comment Organism
drug development essential for virulence, validating this enzyme as a drug target Aspergillus fumigatus

General Stability

General Stability Organism
NADP+, and not NAD+, protects the enzyme from proteolysis Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 NADPH pH 7.5, temperature not specified in the publication Aspergillus fumigatus
0.1
-
 NADH pH 7.5, temperature not specified in the publication Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine + NADH + H+ + O2 Aspergillus fumigatus first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2 Aspergillus fumigatus first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q5SE95
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + NADH + H+ + O2 first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
L-ornithine + NADH + H+ + O2 accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2 first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2 accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
SidA
-
 Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 NADPH pH 7.5, temperature not specified in the publication Aspergillus fumigatus
0.73
-
 NADH pH 7.5, temperature not specified in the publication Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
NADH accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme Aspergillus fumigatus
NADPH accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme. NADP+, and not NAD+, protects the enzyme from proteolysis Aspergillus fumigatus

General Information

General Information Comment Organism
physiological function first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.3
-
 NADH pH 7.5, temperature not specified in the publication Aspergillus fumigatus
50
-
 NADPH pH 7.5, temperature not specified in the publication Aspergillus fumigatus