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Literature summary for 1.13.11.92 extracted from

  • Hammer, A.; Albrecht, F.; Hahne, F.; Jordan, P.; Fraatz, M.; Ley, J.; Geissler, T.; Schrader, J.; Zorn, H.; Buchhaupt, M.
    Biotechnological production of odor-active methyl-branched aldehydes by a novel alpha-dioxygenase from Crocosphaera subtropica (2020), J. Agric. Food Chem., 68, 10432-10440 .
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 1%, doubles the relative enzyme activity Crocosphaera subtropica

Application

Application Comment Organism
synthesis biotechnological production of C12-C15 branched-chain fatty aldehydes. The enzyme efficiently transforms short-chained fatty acids. The transformation products (C12-C15 iso- and anteisoaldehydes) exhibit green-soapy, sweety odors with partial citrus-like, metallic, peppery, and savory-tallowy nuances Crocosphaera subtropica

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Crocosphaera subtropica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0162
-
 13-methyltetradecanoic acid pH 7.5, 25°C Crocosphaera subtropica
0.0263
-
 myristic acid pH 7.5, 25°C Crocosphaera subtropica

Organism

Organism UniProt Comment Textmining
Crocosphaera subtropica B1WSS6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purification by Protino nickel-nitrilotriacetic acid agarose Crocosphaera subtropica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-methyldodecanoic acid + O2
-
 Crocosphaera subtropica (2R)-10-methyl-2-hydroperoxy-dodecanoic acid
-
 ?
11-methyldodecanoic acid + O2
-
 Crocosphaera subtropica (2R)-11-methyl-2-hydroperoxy-dodecanoic acid
-
 ?
11-methyltridecanoic acid + O2
-
 Crocosphaera subtropica (2R)-11-methyl-2-hydroperoxy-tridecanoic acid
-
 ?
12-methyltridecanoic acid + O2
-
 Crocosphaera subtropica (2R)-12-methyl-2-hydroperoxy-tridecanoic acid
-
 ?
13-methylpentadecanoic acid + O2
-
 Crocosphaera subtropica (2R)-13-methyl-2-hydroperoxy-pentadecanoic acid
-
 ?
13-methyltetradecanoic acid + O2
-
 Crocosphaera subtropica (2R)-13-methyl-2-hydroperoxy-tetradecanoic acid
-
 ?
14-methylpentadecanoic acid + O2
-
 Crocosphaera subtropica (2R)-14-methyl-2-hydroperoxy-pentadecanoic acid
-
 ?
myristic acid + O2
-
 Crocosphaera subtropica (2R)-2-hydroperoxy-tetradecanoic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE, x * 69900, calculated from sequence Crocosphaera subtropica

Synonyms

Synonyms Comment Organism
cce_4307
-
 Crocosphaera subtropica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Crocosphaera subtropica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.14
-
 13-methyltetradecanoic acid pH 7.5, 25°C Crocosphaera subtropica
4.03
-
 myristic acid pH 7.5, 25°C Crocosphaera subtropica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Crocosphaera subtropica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
 myristic acid pH 7.5, 25°C Crocosphaera subtropica
200
-
 13-methyltetradecanoic acid pH 7.5, 25°C Crocosphaera subtropica