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Literature summary for 1.13.11.25 extracted from
- 3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase from Nocardia restrictus. I. Isolation of the enzyme and study of its physical and chemical properties (1970), J. Biol. Chem., 245, 5062-5071.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Rhodococcus equi |  |
| 8-hydroxyquinoline | - |
Rhodococcus equi | |
| o-phenanthroline | - |
Rhodococcus equi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 1.13 gatom per mol of enzyme, stimulation of reaction | Rhodococcus equi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 274000 | - |
sedimentation equilibrium | Rhodococcus equi |
| 286000 | - |
sedimentation velocity | Rhodococcus equi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 | Rhodococcus equi | - |
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus equi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone, freezing and thawing, DEAE-cellulose, calcium phosphate cellulose | Rhodococcus equi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22.17 | - |
- |
Rhodococcus equi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10% acetone, relatively long perids, no loss of activity | Rhodococcus equi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 | - |
Rhodococcus equi | 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate | - |
? | |
| 3-methylcatechol | 7.4% of activity with 3-isopropylcatechol | Rhodococcus equi | ? | - |
? | |
| 4-methylcatechol | 4.3% of activity with 3-isopropylcatechol | Rhodococcus equi | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 7.5 | - |
Rhodococcus equi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
inactive below | Rhodococcus equi |
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