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Literature summary for 1.11.1.13 extracted from
- Purification and characterization of two manganese peroxidase isoenzymes from the white-rot basidiomycete Dichomitus squalens (1996), Biochim. Biophys. Acta, 1297, 139-148.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-hydroxy acid | activates by chelating and stabilizing Mn3+ rather than activating the enzyme | Dichomitus squalens |  |
| citrate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
| gluconate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
| glycolate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
| H2O2 | H2O2-dependent | Dichomitus squalens | |
| L-malate | activates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
| L-Tartrate | stimulates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
| Lactate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
| Maleate | slightly activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
| malonate | stimulates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
| additional information | acetate is not an effective chelator | Dichomitus squalens | |
| oxalate | activates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
| phenylacetate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
| phosphate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
| succinate | slightly activates, chelates Mn3+ | Dichomitus squalens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| MnP1 and MnP2 are encoded by different genes | Dichomitus squalens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
Mn2+ | MnP2 | Dichomitus squalens | |
| 0.039 | - |
Mn2+ | MnP1 | Dichomitus squalens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Dichomitus squalens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | expression of MnP isoenzymes is dependent on the presence of Mn, expression rather than enzymatic activity is regulated by Mn | Dichomitus squalens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
MnP1 and MnP2, gel filtration | Dichomitus squalens |
| 48900 | - |
1 * 48000, MnP1, 1 * 48900, MnP2, SDS-PAGE | Dichomitus squalens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | Dichomitus squalens | involved in lignin-degradation | Mn3+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dichomitus squalens | - |
2 isoenzymes: MnP1 and MnP2, syn. Polyporus anceps | - |
| Dichomitus squalens | - |
white rot fungus | - |
| Dichomitus squalens CBS 432.34 | - |
2 isoenzymes: MnP1 and MnP2, syn. Polyporus anceps | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | neutral carbohydrate content: MnP1: 8.5%, MnP2: 10.3% | Dichomitus squalens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 148fold purification of MnP1 and 157fold purification of MnP2 | Dichomitus squalens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 174 | - |
MnP1 | Dichomitus squalens |
| 184 | - |
MnP2 | Dichomitus squalens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
| Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
| Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
| Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
| Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
| Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
| Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
| Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
| Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
| Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
| Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
| Mn2+ + H+ + H2O2 | involved in lignin-degradation | Dichomitus squalens | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens CBS 432.34 | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens CBS 432.34 | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens CBS 432.34 | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens CBS 432.34 | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
| Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens CBS 432.34 | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
| additional information | no oxidation of Co2+ | Dichomitus squalens | ? | - |
? | |
| additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Dichomitus squalens | ? | - |
? | |
| additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Dichomitus squalens | ? | - |
? | |
| additional information | no activity with veratryl alcohol | Dichomitus squalens | ? | - |
? | |
| additional information | no oxidation of Fe2+, Cu2+, Zn2+ | Dichomitus squalens | ? | - |
? | |
| additional information | no other metal can substitute Mn2+ | Dichomitus squalens | ? | - |
? | |
| additional information | enzyme oxidizes 2,6-dimethoxyphenol | Dichomitus squalens | ? | - |
? | |
| additional information | no oxidation of Ni2+ | Dichomitus squalens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 48000, MnP1, 1 * 48900, MnP2, SDS-PAGE | Dichomitus squalens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Dichomitus squalens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
MnP1: pI 4.1, MnP2: pI 3.9 | Dichomitus squalens |
| 4.5 | - |
MnP1 in 50 mM malonate | Dichomitus squalens |
| 5 | - |
MnP2 in 50 mM malonate | Dichomitus squalens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | enzyme contains a pentacoordinated, essentially high-spin ferric heme | Dichomitus squalens | |
| heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Dichomitus squalens | |
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