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Literature summary for 1.1.99.4 extracted from

  • Shinagawa, E.; Ameyama, M.
    2-Keto-D-gluconate dehydrogenase from Gluconobacter melanogenum, membrane-bound (1982), Methods Enzymol., 89, 194-198.
No PubMed abstract available
Search for more literature for 1.1.99.4

General Stability

General Stability Organism
Triton X-100 stabilizes Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
citrate
-
 Gluconobacter oxydans
CN-
-
 Gluconobacter oxydans
additional information not: sulfhydryl reagents Gluconobacter oxydans
oxalate
-
 Gluconobacter oxydans
oxamate
-
 Gluconobacter oxydans
succinate
-
 Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
50
-
 2-dehydro-D-gluconate reaction with ferricyanide Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane outer surface of cytoplasmic membrane Gluconobacter oxydans 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE Gluconobacter oxydans
47000
-
 1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE Gluconobacter oxydans
61000
-
 1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-D-gluconate + acceptor Gluconobacter oxydans enzyme is the primary dehydrogenase of the 2-keto-D-gluconate oxidizing system ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.247
-
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-D-gluconate + acceptor enzyme is the primary dehydrogenase of the 2-keto-D-gluconate oxidizing system Gluconobacter oxydans ?
-
 ?
2-dehydro-D-gluconate + ferricyanide
-
 Gluconobacter oxydans 2,5-didehydro-D-gluconate + ferrocyanide
-
 ?
2-dehydro-D-gluconate + nitro blue tetrazolium
-
 Gluconobacter oxydans 2,5-didehydro-D-gluconate + ?
-
 ?
2-dehydro-D-gluconate + phenazine methosulfate
-
 Gluconobacter oxydans 2,5-didehydro-D-gluconate + ?
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
39
-
-
 Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 stable from pH 5.0 to pH 8.0 Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
cytochrome contains a cytochrome component Gluconobacter oxydans
flavin flavoprotein Gluconobacter oxydans