Literature summary for 1.1.98.2 extracted from

Oyugi, M.A.; Bashiri, G.; Baker, E.N.; Johnson-Winters, K.
Investigating the reaction mechanism of F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium tuberculosis: Kinetic analysis of the wild-type and mutant enzymes (2016), Biochemistry, 55, 5566-5577.

Search for more literature for 1.1.98.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Top10 cells	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
E109Q	the mutant has 60fold decreased catalytic efficiency compared to the wild type enzyme	Mycobacterium tuberculosis
H40A	the mutant has 1000fold decreased catalytic efficiency compared to the wild type enzyme	Mycobacterium tuberculosis
W44A	the mutant has 2000fold decreased catalytic efficiency compared to the wild type enzyme	Mycobacterium tuberculosis
W44F	the mutant has 2fold increased catalytic efficiency compared to the wild type enzyme	Mycobacterium tuberculosis
W44Y	the mutant has 34fold decreased catalytic efficiency compared to the wild type enzyme	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0009	-	oxidized coenzyme F420	mutant enzyme W44A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.0026	-	oxidized coenzyme F420	wild type enzyme, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.0042	-	oxidized coenzyme F420	mutant enzyme W44Y, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.0054	-	oxidized coenzyme F420	mutant enzyme W44F, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.0056	-	oxidized coenzyme F420	mutant enzyme E109Q, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.008	-	oxidized coenzyme F420	mutant enzyme H40A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.022	-	D-glucose 6-phosphate	wild type enzyme, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.027	-	D-glucose 6-phosphate	mutant enzyme H40A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.041	-	D-glucose 6-phosphate	mutant enzyme E109Q, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.17	-	D-glucose 6-phosphate	mutant enzyme W44Y, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.28	-	D-glucose 6-phosphate	mutant enzyme W44A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.34	-	D-glucose 6-phosphate	mutant enzyme W44F, at pH 7.0 and 22°C	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose 6-phosphate + oxidized coenzyme F420	Mycobacterium tuberculosis	-	6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	Q0RVH7	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Sephacryl 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate + oxidized coenzyme F420	-	Mycobacterium tuberculosis	6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420	-	?

Synonyms

Synonyms	Comment	Organism
F420-dependent glucose-6-phosphate dehydrogenase	-	Mycobacterium tuberculosis
FGD	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.001	-	oxidized coenzyme F420	mutant enzyme W44A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.0012	-	D-glucose 6-phosphate	mutant enzyme W44A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.0016	-	D-glucose 6-phosphate	mutant enzyme H40A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.002	-	oxidized coenzyme F420	mutant enzyme H40A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.02	-	oxidized coenzyme F420	mutant enzyme E109Q, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.023	-	D-glucose 6-phosphate	mutant enzyme E109Q, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.041	-	D-glucose 6-phosphate	mutant enzyme W44Y, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.06	-	oxidized coenzyme F420	mutant enzyme W44Y, at pH 7.0 and 22°C	Mycobacterium tuberculosis
1.4	-	D-glucose 6-phosphate	wild type enzyme, at pH 7.0 and 22°C	Mycobacterium tuberculosis
1.4	-	oxidized coenzyme F420	wild type enzyme, at pH 7.0 and 22°C	Mycobacterium tuberculosis
3.2	-	oxidized coenzyme F420	mutant enzyme W44F, at pH 7.0 and 22°C	Mycobacterium tuberculosis
3.5	-	D-glucose 6-phosphate	mutant enzyme W44F, at pH 7.0 and 22°C	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
coenzyme F420	-	Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.004	-	D-glucose 6-phosphate	mutant enzyme W44A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.06	-	D-glucose 6-phosphate	mutant enzyme H40A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.1	-	D-glucose 6-phosphate	mutant enzyme E109Q, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.2	-	D-glucose 6-phosphate	mutant enzyme W44Y, at pH 7.0 and 22°C	Mycobacterium tuberculosis
0.4	-	oxidized coenzyme F420	mutant enzyme H40A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
1	-	oxidized coenzyme F420	mutant enzyme W44A, at pH 7.0 and 22°C	Mycobacterium tuberculosis
4	-	oxidized coenzyme F420	mutant enzyme E109Q, at pH 7.0 and 22°C	Mycobacterium tuberculosis
10	-	D-glucose 6-phosphate	mutant enzyme W44F, at pH 7.0 and 22°C	Mycobacterium tuberculosis
20	-	oxidized coenzyme F420	mutant enzyme W44Y, at pH 7.0 and 22°C	Mycobacterium tuberculosis
60	-	D-glucose 6-phosphate	wild type enzyme, at pH 7.0 and 22°C	Mycobacterium tuberculosis
600	-	oxidized coenzyme F420	wild type enzyme, at pH 7.0 and 22°C	Mycobacterium tuberculosis
600	-	oxidized coenzyme F420	mutant enzyme W44F, at pH 7.0 and 22°C	Mycobacterium tuberculosis

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Release 2026.1 (March 2026)