Literature summary for 1.1.98.2 extracted from

Bashiri, G.; Squire, C.J.; Moreland, N.J.; Baker, E.N.
Crystal structures of F420-dependent glucose-6-phosphate dehydrogenase FGD1 involved in the activation of the anti-tuberculosis drug candidate PA-824 reveal the basis of coenzyme and substrate binding (2008), J. Biol. Chem., 283, 17531-17541.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of the enzyme is determined by X-ray crystallography both in its apo state and in complex with F420 and citrate at resolutions of 1.90 and 1.95 A, respectively. The structure reveals a highly specific F420 binding mode, which is shared with several other F420-dependent enzymes. The competitive inhibitor citrate occupies the substrate binding pocket adjacent to F420. Modeling of the binding of the glucose 6-phosphate substrate identifies a positively charged phosphate binding pocket and shows that glucose 6-phosphate, like citrate, packs against the isoalloxazine moiety of F420 and helps promote a butterfly bend conformation that facilitates F420 reduction and catalysis	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

structure of the enzyme is determined by X-ray crystallography both in its apo state and in complex with F420 and citrate at resolutions of 1.90 and 1.95 A, respectively. The structure reveals a highly specific F420 binding mode, which is shared with several other F420-dependent enzymes. The competitive inhibitor citrate occupies the substrate binding pocket adjacent to F420. Modeling of the binding of the glucose 6-phosphate substrate identifies a positively charged phosphate binding pocket and shows that glucose 6-phosphate, like citrate, packs against the isoalloxazine moiety of F420 and helps promote a butterfly bend conformation that facilitates F420 reduction and catalysis

Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
citrate	competitive	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WNE1	-	-
Mycobacterium tuberculosis H37Rv	P9WNE1	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycobacterium tuberculosis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.043	-	-	Mycobacterium tuberculosis	citrate

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Release 2026.1 (March 2026)