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Literature summary for 1.1.3.30 extracted from

  • Kawagoshi, Y.; Fujita, M.
    Purification and properties of polyvinyl alcohol oxidase with broad substrate range obtained from Pseudomonas vesicularis var. povalolyticus PH (1997), World J. Microbiol. Biotechnol., 13, 273-277.
No PubMed abstract available
Search for more literature for 1.1.3.30

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 23% inhibition Brevundimonas vesicularis
Fe2+ 1 mM, 88% inhibition Brevundimonas vesicularis
Hg2+ 1 mM, 93% inhibition Brevundimonas vesicularis
Sn2+ 1 mM, 89% inhibition Brevundimonas vesicularis
Thiourea 1 mM, 20% inhibition Brevundimonas vesicularis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 mol iron/mol enzyme Brevundimonas vesicularis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration Brevundimonas vesicularis
85000
-
 1 * 85000, SDS-PAGE Brevundimonas vesicularis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polyvinyl alcohol + O2 Brevundimonas vesicularis
-
 oxidized polyvinyl alcohol + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Brevundimonas vesicularis
-
 var. povalolyticus PH
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, AF blue Toyopearl, Q-Sepharose Brevundimonas vesicularis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
-
 Brevundimonas vesicularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-butanediol + O2 3.8% of activity with polyvinyl alcohol Brevundimonas vesicularis 1,3-butanedione + H2O2
-
 ?
2,4-pentanediol + O2 19.3% of activity with polyvinyl alcohol Brevundimonas vesicularis 2,4-pentanedione + H2O2
-
 ?
2,5-hexanediol + O2 3.8% of activity with polyvinyl alcohol Brevundimonas vesicularis 2,5-hexanedione + H2O2
-
 ?
2-butanol + O2 47.3% of activity with polyvinyl alcohol Brevundimonas vesicularis 2-butanone + H2O2
-
 ?
2-hexanol + O2 65.4% of activity with polyvinyl alcohol Brevundimonas vesicularis 2-hexanone + H2O2
-
 ?
2-pentanol + O2 36.3% of activity with polyvinyl alcohol Brevundimonas vesicularis 2-pentanone + H2O2
-
 ?
2-propanol + O2 3.5% of activity with polyvinyl alcohol Brevundimonas vesicularis acetone + H2O2
-
 ?
3-butanol + O2 14.6% of activity with polyvinyl alcohol Brevundimonas vesicularis 3-butanone + H2O2
-
 ?
3-heptanol + O2 68.8% of activity with polyvinyl alcohol Brevundimonas vesicularis 3-heptanone + H2O2
-
 ?
3-hexanol + O2 47.3% of activity with polyvinyl alcohol Brevundimonas vesicularis 3-hexanone + H2O2
-
 ?
4-decanol + O2 100% of activity with polyvinyl alcohol Brevundimonas vesicularis 4-decanone + H2O2
-
 ?
4-heptanol + O2 75.1% of activity with polyvinyl alcohol Brevundimonas vesicularis 4-heptanone + H2O2
-
 ?
benzyl alcohol + O2 13.7% of activity with polyvinyl alcohol Brevundimonas vesicularis benzaldehyde + H2O2
-
 ?
cyclohexanol + O2 41.8% of activity with polyvinyl alcohol Brevundimonas vesicularis cyclohexanal + H2O2
-
 ?
polyvinyl alcohol + O2
-
 Brevundimonas vesicularis oxidized polyvinyl alcohol + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 85000, SDS-PAGE Brevundimonas vesicularis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Brevundimonas vesicularis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 45
-
 Brevundimonas vesicularis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable for 30 min, complete loss of activity after 30 min at 60°C Brevundimonas vesicularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Brevundimonas vesicularis

pH Range

pH Minimum pH Maximum Comment Organism
7 11 almost constant activity in this range Brevundimonas vesicularis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 stable for at least 24 h at 30°C Brevundimonas vesicularis