General Stability | Organism |
---|---|
after solubilization the enzyme gradually loses activity when kept at 5°C, full reactivation by freezing and thawing | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Dinitrophenylhydrazine | - |
Pseudomonas putida | |
2-mercaptoethanol | - |
Pseudomonas putida | |
8-hydroxyquinoline | - |
Pseudomonas putida | |
Ag+ | - |
Pseudomonas putida | |
Atebrin | - |
Pseudomonas putida | |
Co2+ | - |
Pseudomonas putida | |
Cu2+ | - |
Pseudomonas putida | |
cysteine | - |
Pseudomonas putida | |
diethyldithiocarbamate | - |
Pseudomonas putida | |
dithiothreitol | - |
Pseudomonas putida | |
DL-3,4-dihydroxymandelic acid | - |
Pseudomonas putida | |
EDTA | - |
Pseudomonas putida | |
Fe2+ | - |
Pseudomonas putida | |
Fe3+ | - |
Pseudomonas putida | |
guanidine hydrochloride | - |
Pseudomonas putida | |
Hg2+ | - |
Pseudomonas putida | |
hydroxylamine | - |
Pseudomonas putida | |
potassium cyanide | - |
Pseudomonas putida | |
reduced glutathione | - |
Pseudomonas putida | |
SDS | - |
Pseudomonas putida | |
Zn2+ | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
DL-4-hydroxymandelate | - |
Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Pseudomonas putida | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 | Pseudomonas putida | enzyme of degradation pathway from mandelic acid to benzoic acid | 4-hydroxybenzaldehyde + CO2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, 2 months, solubilized enzyme | Pseudomonas putida |
-20°C, particulate fraction suspended in 0.025 M sodium phosphate buffer, pH 7.0, more than 6 months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 | absolute requirement for molecular oxygen, specific for L-isomer of 4-hydroxymandelic acid | Pseudomonas putida | 4-hydroxybenzaldehyde + CO2 + H2O2 | - |
? | |
(S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 | enzyme of degradation pathway from mandelic acid to benzoic acid | Pseudomonas putida | 4-hydroxybenzaldehyde + CO2 + H2O2 | - |
? | |
DL-4-hydroxymandelate + ? | - |
Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-4-hydroxymandelate oxidase (decarboxylating) | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required | Pseudomonas putida |