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Literature summary for 1.1.2.7 extracted from
- The quinoprotein dehydrogenases for methanol and glucose (2004), Arch. Biochem. Biophys., 428, 2-9.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure determination | Methylophilus sp. |
| X-ray diffraction structure determination | Methylorubrum extorquens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state analysis using stopped-flow kinetics, molecular dynamics, overview | Methylophilus sp. | |
| additional information | - |
additional information | steady-state analysis using stopped-flow kinetics, molecular dynamics, overview | Methylorubrum extorquens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Methylophilus sp. | - |
- |
| periplasm | - |
Methylorubrum extorquens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | can substitute for Ca2+, Ba-MDH has twice the maximum activity of the Ca-MDH but with a much lower affinity for its substrates | Methylorubrum extorquens |  |
| Ca2+ | the active site contains a single Ca2+ whose coordination sphere contains PQQ and protein atoms | Methylophilus sp. | |
| Ca2+ | the active site contains a single Ca2+ whose coordination sphere contains PQQ and protein atoms | Methylorubrum extorquens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 8500 | - |
2 * 66000 + 2 * 8500, alpha2beta2, crystal structure determination | Methylorubrum extorquens |
| 66000 | - |
2 * 66000 + 2 * 8500, alpha2beta2, crystal structure determination | Methylorubrum extorquens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methanol + ferricytochrome cL | Methylophilus sp. | MDH catalyses the first reaction of an unusual periplasmic electron transport chain responsible for oxidation of methanol to formaldehyde in methylotrophic bacteria during growth on methane or methanol | formaldehyde + ferrocytochrome cL | - |
? | |
| methanol + ferricytochrome cL | Methylorubrum extorquens | MDH catalyses the first reaction of an unusual periplasmic electron transport chain responsible for oxidation of methanol to formaldehyde in methylotrophic bacteria during growth on methane or methanol | formaldehyde + ferrocytochrome cL | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylophilus sp. | - |
- |
- |
| Methylorubrum extorquens | P16027 and P14775 | P16027 (large subunit, alpha) and P14775 (small subunit, beta) | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+ | detailed reaction mechanism with direct hydride transfe, Glu177 plays the role of general base catalyst | Methylorubrum extorquens | |
| a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+ | detailed reaction mechanism with direct hydride transfer | Methylophilus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methanol + ferricytochrome cL | - |
Methylophilus sp. | formaldehyde + ferrocytochrome cL | - |
? | |
| methanol + ferricytochrome cL | - |
Methylorubrum extorquens | formaldehyde + ferrocytochrome cL | - |
? | |
| methanol + ferricytochrome cL | MDH catalyses the first reaction of an unusual periplasmic electron transport chain responsible for oxidation of methanol to formaldehyde in methylotrophic bacteria during growth on methane or methanol | Methylophilus sp. | formaldehyde + ferrocytochrome cL | - |
? | |
| methanol + ferricytochrome cL | MDH catalyses the first reaction of an unusual periplasmic electron transport chain responsible for oxidation of methanol to formaldehyde in methylotrophic bacteria during growth on methane or methanol | Methylorubrum extorquens | formaldehyde + ferrocytochrome cL | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the large alpha-subunit has a propeller fold making up a superbarrel of eight radially arranged beta-sheets, i.e. the propeller blades, containing the tryptophan-docking motifs that link together the eight beta-sheets, and the presence in the active site of a unique eight-membered disulfide ring structure formed from adjacent cysteine residues 103 and 104, joined by an atypical non-planar peptide bond | Methylorubrum extorquens |
| tetramer | 2 * 66000 + 2 * 8500, alpha2beta2, crystal structure determination | Methylorubrum extorquens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methanol dehydrogenase | - |
Methylophilus sp. |
| methanol dehydrogenase | - |
Methylorubrum extorquens |
| quinoprotein dehydrogenase | - |
Methylophilus sp. |
| quinoprotein dehydrogenase | - |
Methylorubrum extorquens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Methylorubrum extorquens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome cL | - |
Methylophilus sp. | |
| cytochrome cL | - |
Methylorubrum extorquens | |
| pyrroloquinoline quinone | PQQ, is the only prosthetic group | Methylophilus sp. | |
| pyrroloquinoline quinone | PQQ, is the only prosthetic group, the PQQ is sandwiched between the indole ring of Trp243 and the disulfide ring structure, overview | Methylorubrum extorquens | |
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