Literature summary for 1.1.2.7 extracted from

Liu, Q.; Kirchhoff, J.R.; Faehnle, C.R.; Viola, R.E.; Hudson, R.A.
A rapid method for the purification of methanol dehydrogenase from Methylobacterium extorquens (2006), Protein Expr. Purif., 46, 316-320.

Search for more literature for 1.1.2.7

General Stability

General Stability	Organism
EDTA, DTT, and Ca2+ are slightly stabilizing	Methylorubrum extorquens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	bound to the active site, required for catalytic activity	Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
7500	-	x * 62000, alpha-subunit, + x * 7500, beta-subunit, SDS-PAGE	Methylorubrum extorquens
62000	-	x * 62000, alpha-subunit, + x * 7500, beta-subunit, SDS-PAGE	Methylorubrum extorquens

Organism

Organism	UniProt	Comment	Textmining
Methylorubrum extorquens	P16027 and P14775	P16027 (large subunit, alpha) and P14775 (small subunit, beta)	-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1	P16027 and P14775	P16027 (large subunit, alpha) and P14775 (small subunit, beta)	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 22fold from strain AM1 in a single cation exchange chromatographical step, followed by ultrafiltration and buffer exchange, over 97% purity	Methylorubrum extorquens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.4	-	purified enzyme	Methylorubrum extorquens

Storage Stability

Storage Stability	Organism
-80°C, purified native enzyme, 20 mM phosphate, pH 7.0, negligible loss of activity in 6 months	Methylorubrum extorquens
4°C, purified native enzyme, 20 mM phosphate, pH 7.0, loss of 80% activity within 1 week	Methylorubrum extorquens

Subunits

Subunits	Comment	Organism
?	x * 62000, alpha-subunit, + x * 7500, beta-subunit, SDS-PAGE	Methylorubrum extorquens

Synonyms

Synonyms	Comment	Organism
MDH	-	Methylorubrum extorquens
methanol dehydrogenase	-	Methylorubrum extorquens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	assay at	Methylorubrum extorquens

Cofactor

Cofactor	Comment	Organism	Structure
pyrroloquinoline quinone	i.e. PQQ or 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylaic acid, enzyme-bound to the active site, 1 molecule per alpha-subunit, required for catalytic activity	Methylorubrum extorquens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Methylorubrum extorquens	sequence calculation	-	8.8

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Release 2023.1 (January 2023)