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Literature summary for 1.1.2.4 extracted from

  • Smutok, O.V.; Dmytruk, K.V.; Karkovska, M.I.; Schuhmann, W.; Gonchar, M.V.; Sibirny, A.A.
    D-Lactate-selective amperometric biosensor based on the cell debris of the recombinant yeast Hansenula polymorpha (2014), Talanta, 125, 227-232 .
    View publication on PubMed
Search for more literature for 1.1.2.4

Cloned(Commentary)

Cloned (Comment) Organism
gene DLD1, recombinant expression in L-lactate-cytochrome c oxidoreductase CYB2 deficient Hansenula polymorpha strain NCYC495 leu1-1 is used for recombinant overexpression of the His6-tagged enzyme using the gene with a terminator region driven by the alcohol oxidase gene promoter. The mutant strain shows increased DlDH activity and abolished activity of flavocytochrome b2, EC 1.1.2.3 Ogataea angusta

Protein Variants

Protein Variants Comment Organism
additional information construction and evaluation of amperometric cell debris-based biosensors using constant-potential amperometry in a three-electrode configuration with an Ag/AgCl/KCl(3M) reference electrode and a Pt-wire counter-electrode. The strain DELTAcyb2/DLD1 is a suitable biorecognition element for the construction of biosensors selective for D-lactate, overview Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + 2 ferricytochrome c Ogataea angusta
-
 pyruvate + 2 ferrocytochrome c
-
 ?
(R)-lactate + 2 ferricytochrome c Ogataea angusta tr6
-
 pyruvate + 2 ferrocytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Ogataea angusta W1QLN6
-
-
Ogataea angusta tr6 W1QLN6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + 2 ferricytochrome c
-
 Ogataea angusta pyruvate + 2 ferrocytochrome c
-
 ?
(R)-lactate + 2 ferricytochrome c
-
 Ogataea angusta tr6 pyruvate + 2 ferrocytochrome c
-
 ?

Synonyms

Synonyms Comment Organism
DLD1
-
 Ogataea angusta
DLDH
-
 Ogataea angusta
L-lactate-cytochrome c oxidoreductase
-
 Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
 Ogataea angusta