Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-CodonPlus RIL cells | Gluconobacter oxydans |
General Stability | Organism |
---|---|
stability of the enzyme significantly improves (up to 13.6fold) after cross-linking of immobilized enzyme on silica nanoparticles and retains 62.8% residual activity after 10 cycles of reuse | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-sorbitol | inhibitory at more than 10% (w/v) | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.9 | - |
D-sorbitol | at pH 10.0 and 25°C | Gluconobacter oxydans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Gluconobacter oxydans | |
Ca2+ | activates | Gluconobacter oxydans | |
Cu2+ | activates | Gluconobacter oxydans | |
K+ | activates | Gluconobacter oxydans | |
additional information | Mg2+, Co2+, and Mn2+ ions show no stimulatory influence on enzyme activity | Gluconobacter oxydans | |
Zn2+ | activates | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Gluconobacter oxydans G624 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinitol + NADP+ | - |
Gluconobacter oxydans | ? | - |
? | |
D-sorbitol + NADP+ | best substrate | Gluconobacter oxydans | L-sorbose + NADPH + H+ | - |
? | |
D-sorbitol + NADP+ | best substrate | Gluconobacter oxydans G624 | L-sorbose + NADPH + H+ | - |
? | |
mannitol + NADP+ | - |
Gluconobacter oxydans | ? | - |
? | |
additional information | no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol | Gluconobacter oxydans | ? | - |
? | |
additional information | no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol | Gluconobacter oxydans G624 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 54000, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
D-sorbitol dehydrogenase (NADP+) | - |
Gluconobacter oxydans |
D-sorbitol:NADP+ oxidoreductase (L-sorbose-forming) | - |
Gluconobacter oxydans |
SLDH | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Gluconobacter oxydans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | more than 40% activity between 25 and 75°C | Gluconobacter oxydans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3820 | - |
D-sorbitol | at pH 10.0 and 25°C | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme exhibits high preference for NADP+ (vs. 2.5% relative activity with NAD+) | Gluconobacter oxydans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
98.1 | - |
D-sorbitol | at pH 10.0 and 25°C | Gluconobacter oxydans |