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Literature summary for 1.1.1.93 extracted from

  • Giffhorn, F.; Kuhn, A.
    Purification and characterization of a bifunctional L-(+)-tartrate dehydrogenase-D-(+)-malate dehydrogenase (decarboxylating) from Rhodopseudomonas sphaeroides Y (1983), J. Bacteriol., 155, 281-290.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NH4+ monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM Cereibacter sphaeroides

General Stability

General Stability Organism
repeated freezing and thawing of purified enzyme, 10-40% loss of activity Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Cereibacter sphaeroides
Dihydroxyfumarate
-
Cereibacter sphaeroides
meso-tartrate competitive Cereibacter sphaeroides
oxaloacetate competitive Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
NAD+ reaction with D-malate Cereibacter sphaeroides
0.17
-
D-malate
-
Cereibacter sphaeroides
0.28
-
NAD+ reaction with (+ L-(+)-tartrate) Cereibacter sphaeroides
2.3
-
L-Tartrate
-
Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 30% of the activation with 0.4 mM MnCl2 Cereibacter sphaeroides
K+ 50 mM KCl, 63% of the activation with 50 mM NH4Cl Cereibacter sphaeroides
K+ 50 mM K2SO4, 56% of the activation with 50 mM KCl Cereibacter sphaeroides
Mg2+ 5 mM, 59% of the activation with 0.4 mM MnCl2 Cereibacter sphaeroides
Mn2+ Km: 0.016 mM, reaction with L-malate or D-malate Cereibacter sphaeroides
Mn2+ monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM Cereibacter sphaeroides
NaCl 50 mM KCl, 19% of the activation with 50 mM NH4Cl Cereibacter sphaeroides
Zn2+ 0.1 mM, 14% of the activation with 0.4 mM MnCl2 Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
4 * 38500, SDS-PAGE Cereibacter sphaeroides
158000
-
gel filtration Cereibacter sphaeroides
162000
-
ultracentrifugation Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
bifunctional L-(+)-tartrate dehydrogenase/D-(+)-malate dehydrogenase (decarboxylating), EC 1.1.1.93/EC 1.1.1.83 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.88
-
-
Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-20°C, months, partially purified enzyme Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-malate + NAD+
-
Cereibacter sphaeroides pyruvate + CO2 + NADH + H+
-
r
D-malate + NAD+
-
Cereibacter sphaeroides Y pyruvate + CO2 + NADH + H+
-
r
L-tartrate + NAD+
-
Cereibacter sphaeroides oxaloglycolate + NADH + H+ dihydroxyfumarate is in tautomeric equilibrium with oxaloglycolate ?
L-tartrate + NAD+
-
Cereibacter sphaeroides Y oxaloglycolate + NADH + H+ dihydroxyfumarate is in tautomeric equilibrium with oxaloglycolate ?

Subunits

Subunits Comment Organism
tetramer 4 * 38500, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 7.0, 30 h: 50% loss of activity. pH 8.5, stable for 4 h Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 9 bifunctional L-(+)-tartrate dehydrogenase/D-(+)-malate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 Cereibacter sphaeroides

pH Range

pH Minimum pH Maximum Comment Organism
7 10.6 at pH 7.0 and pH 10.6: 50% of activity maximum Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NAD+ cofactor Cereibacter sphaeroides