Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM | Cereibacter sphaeroides |
General Stability | Organism |
---|---|
repeated freezing and thawing of purified enzyme, 10-40% loss of activity | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Cereibacter sphaeroides | |
Dihydroxyfumarate | - |
Cereibacter sphaeroides | |
meso-tartrate | competitive | Cereibacter sphaeroides | |
oxaloacetate | competitive | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NAD+ | reaction with D-malate | Cereibacter sphaeroides | |
0.17 | - |
D-malate | - |
Cereibacter sphaeroides | |
0.28 | - |
NAD+ | reaction with (+ L-(+)-tartrate) | Cereibacter sphaeroides | |
2.3 | - |
L-Tartrate | - |
Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 30% of the activation with 0.4 mM MnCl2 | Cereibacter sphaeroides | |
K+ | 50 mM KCl, 63% of the activation with 50 mM NH4Cl | Cereibacter sphaeroides | |
K+ | 50 mM K2SO4, 56% of the activation with 50 mM KCl | Cereibacter sphaeroides | |
Mg2+ | 5 mM, 59% of the activation with 0.4 mM MnCl2 | Cereibacter sphaeroides | |
Mn2+ | Km: 0.016 mM, reaction with L-malate or D-malate | Cereibacter sphaeroides | |
Mn2+ | monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM | Cereibacter sphaeroides | |
NaCl | 50 mM KCl, 19% of the activation with 50 mM NH4Cl | Cereibacter sphaeroides | |
Zn2+ | 0.1 mM, 14% of the activation with 0.4 mM MnCl2 | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
158000 | - |
gel filtration | Cereibacter sphaeroides |
162000 | - |
ultracentrifugation | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
bifunctional L-(+)-tartrate dehydrogenase/D-(+)-malate dehydrogenase (decarboxylating), EC 1.1.1.93/EC 1.1.1.83 | Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.88 | - |
- |
Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
-20°C, months, partially purified enzyme | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-malate + NAD+ | - |
Cereibacter sphaeroides | pyruvate + CO2 + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Cereibacter sphaeroides Y | pyruvate + CO2 + NADH + H+ | - |
r | |
L-tartrate + NAD+ | - |
Cereibacter sphaeroides | oxaloglycolate + NADH + H+ | dihydroxyfumarate is in tautomeric equilibrium with oxaloglycolate | ? | |
L-tartrate + NAD+ | - |
Cereibacter sphaeroides Y | oxaloglycolate + NADH + H+ | dihydroxyfumarate is in tautomeric equilibrium with oxaloglycolate | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cereibacter sphaeroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.0, 30 h: 50% loss of activity. pH 8.5, stable for 4 h | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 9 | bifunctional L-(+)-tartrate dehydrogenase/D-(+)-malate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 | Cereibacter sphaeroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.6 | at pH 7.0 and pH 10.6: 50% of activity maximum | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Cereibacter sphaeroides |