KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADPH | 2 mM 2,3-dihydroxysuccinate as substrate | Pseudomonas putida | |
0.024 | - |
NADPH | 2 mM hydroxypyruvate as substrate | Pseudomonas putida | |
0.14 | - |
NADH | 2 mM 2,3-dihydroxysuccinate as substrate | Pseudomonas putida | |
0.17 | - |
NADH | 2 mM hydroxypyruvate as substrate | Pseudomonas putida | |
1 | - |
Hydroxypyruvate | cofactor NADH | Pseudomonas putida | |
1.2 | - |
2,3-dihydroxysuccinate | cofactor NADH | Pseudomonas putida | |
2.5 | - |
Hydroxypyruvate | cofactor NADPH | Pseudomonas putida | |
2.5 | - |
2,3-dihydroxysuccinate | cofactor NADPH | Pseudomonas putida | |
3.3 | - |
glyoxylate | cofactor NADH | Pseudomonas putida | |
8 | - |
glyoxylate | cofactor NADPH | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
sedimentation equilibrium | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-3-oxosuccinate + NAD(P)H | Pseudomonas putida | - |
D-glycerate + NAD(P)+ + CO2 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-100, disc electrophoresis, Sephadex G-100, crystallization | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
substrate 2,3-dihydroxy-succinate | Pseudomonas putida |
7.7 | - |
substrate hydroxypyruvate | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-15°C, 40 mM Tris-HCl, 30% glycerol, 5 mM mercaptoethanol, 6 months, 10% loss of activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxysuccinate + NAD(P)H | - |
Pseudomonas putida | D-glycerate + NAD(P)+ + CO2 | - |
ir | |
2-hydroxy-3-oxosuccinate + NAD(P)H | - |
Pseudomonas putida | D-glycerate + NAD(P)+ + CO2 | - |
r | |
2-hydroxy-3-oxosuccinate + NAD(P)H | enzyme is inhibited at higher levels of ionic strength | Pseudomonas putida | D-glycerate + NAD(P)+ + CO2 | - |
r | |
2-hydroxy-3-oxosuccinate + NADH + H+ | - |
Pseudomonas putida | D-glycerate + NAD+ + CO2 | - |
r | |
glyoxylate + NAD(P)H | - |
Pseudomonas putida | glycolic acid + NAD(P)+ | - |
ir | |
hydroxypyruvate + NAD(P)H | - |
Pseudomonas putida | D-glycerate + NAD(P)+ + H+ | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Pseudomonas putida |