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Literature summary for 1.1.1.90 extracted from

  • Nishimura, M.
    Molecular cloning and expression of the Streptomyces coniferyl alcohol dehydrogenase gene in Escherichia coli (2013), Protein Expr. Purif., 89, 109-115.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, functional recombinant enzyme overexpression in Escherichia coli strain BL21(DE3), optimization of conditions and method for soluble CADH expression, expression of active CADH under optimized conditions is approximately 4fold higher than in the absence of heat shock, solution of inclusion body formation by heat-shock treatment of recombinant Escherichia coli carrying the Streptomyces CADH gene Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
coniferyl alcohol native enzyme, pH 8.5, 30°C Streptomyces sp.
0.28
-
coniferyl alcohol recombinant enzyme, pH 8.5, 30°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
recombinant enzyme, gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coniferyl alcohol + NAD+ Streptomyces sp. CADH requires NAD as a cofactor coniferyl aldehyde + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. M5AJW4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme about 40fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, hydrophobic interaction chromatography, again dialysis, and anion exchange chromatography Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.7
-
puriifed recombinant enzyme, pH 8.5, 30°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coniferyl alcohol + NAD+ CADH requires NAD as a cofactor Streptomyces sp. coniferyl aldehyde + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4* 39000, recombinant and native enzyme, SDS-PAGE, 4* 37845, sequence calculation Streptomyces sp.

Synonyms

Synonyms Comment Organism
CADH
-
Streptomyces sp.
coniferyl alcohol dehydrogenase
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25.8
-
coniferyl alcohol recombinant enzyme, pH 8.5, 30°C Streptomyces sp.
69.7
-
coniferyl alcohol native enzyme, pH 8.5, 30°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Streptomyces sp.

General Information

General Information Comment Organism
evolution the enzyme belongs to the medium-chain zinc-dependent alcohol dehydrogenases Streptomyces sp.
physiological function the enzyme is a medium-chain zinc-dependent alcohol dehydrogenase Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
93.3
-
coniferyl alcohol recombinant enzyme, pH 8.5, 30°C Streptomyces sp.
536
-
coniferyl alcohol native enzyme, pH 8.5, 30°C Streptomyces sp.