Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 1 mM, 30% residual activity | Streptomyces sp. | |
iodoacetic acid | 1 mM, 57% residual activity | Streptomyces sp. | |
N-bromosuccinimide | 1 mM, 3% residual activity | Streptomyces sp. | |
Zn2+ | 1 mM, 9% residual activity | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
coniferyl alcohol | pH 8.0, 30°C | Streptomyces sp. | |
0.4 | - |
NAD+ | pH 8.0, 30°C | Streptomyces sp. | |
1.7 | - |
cinnamyl alcohol | pH 8.0, 30°C | Streptomyces sp. | |
5.1 | - |
coniferyl aldehyde | pH 8.0, 30°C | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37598 | - |
4 * 37598, MALDI-TOF | Streptomyces sp. |
151000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | M5AJW4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamic aldehyde + NADH + H+ | - |
Streptomyces sp. | cinnamyl alcohol + NAD+ | - |
r | |
cinnamyl alcohol + NAD+ | - |
Streptomyces sp. | cinnamic aldehyde + NADH + H+ | - |
r | |
coniferyl alcohol + NAD+ | - |
Streptomyces sp. | coniferyl aldehyde + NADH + H+ | - |
r | |
coniferyl aldehyde + NADH + H+ | - |
Streptomyces sp. | coniferyl alcohol + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37598, MALDI-TOF | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
CADH II | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.0, stable up to | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
53.7 | - |
NAD+ | pH 8.0, 30°C | Streptomyces sp. | |
69.7 | - |
coniferyl alcohol | pH 8.0, 30°C | Streptomyces sp. | |
116 | - |
cinnamyl alcohol | pH 8.0, 30°C | Streptomyces sp. | |
354 | - |
coniferyl aldehyde | pH 8.0, 30°C | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | 75 h, more than 80% of initial activity | Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADPH | Streptomyces sp. | |
NAD+ | - |
Streptomyces sp. | |
NADH | - |
Streptomyces sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
68.3 | - |
cinnamyl alcohol | pH 8.0, 30°C | Streptomyces sp. | |
69.3 | - |
coniferyl aldehyde | pH 8.0, 30°C | Streptomyces sp. | |
134 | - |
NAD+ | pH 8.0, 30°C | Streptomyces sp. | |
536 | - |
coniferyl alcohol | pH 8.0, 30°C | Streptomyces sp. |