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Literature summary for 1.1.1.90 extracted from

  • Nishimura, M.; Kohno, K.; Nishimura, Y.; Inagaki, M.; Davies, J.
    Characterization of two isozymes of coniferyl alcohol dehydrogenase from Streptomyces sp. NL15-2K (2011), Biosci. Biotechnol. Biochem., 75, 1770-1777.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ 1 mM, 30% residual activity Streptomyces sp.
iodoacetic acid 1 mM, 57% residual activity Streptomyces sp.
N-bromosuccinimide 1 mM, 3% residual activity Streptomyces sp.
Zn2+ 1 mM, 9% residual activity Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
coniferyl alcohol pH 8.0, 30°C Streptomyces sp.
0.4
-
NAD+ pH 8.0, 30°C Streptomyces sp.
1.7
-
cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
5.1
-
coniferyl aldehyde pH 8.0, 30°C Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37598
-
4 * 37598, MALDI-TOF Streptomyces sp.
151000
-
gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. M5AJW4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamic aldehyde + NADH + H+
-
Streptomyces sp. cinnamyl alcohol + NAD+
-
r
cinnamyl alcohol + NAD+
-
Streptomyces sp. cinnamic aldehyde + NADH + H+
-
r
coniferyl alcohol + NAD+
-
Streptomyces sp. coniferyl aldehyde + NADH + H+
-
r
coniferyl aldehyde + NADH + H+
-
Streptomyces sp. coniferyl alcohol + NAD+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 37598, MALDI-TOF Streptomyces sp.

Synonyms

Synonyms Comment Organism
CADH II
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 7.0, stable up to Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
53.7
-
NAD+ pH 8.0, 30°C Streptomyces sp.
69.7
-
coniferyl alcohol pH 8.0, 30°C Streptomyces sp.
116
-
cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
354
-
coniferyl aldehyde pH 8.0, 30°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 75 h, more than 80% of initial activity Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADPH Streptomyces sp.
NAD+
-
Streptomyces sp.
NADH
-
Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
68.3
-
cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
69.3
-
coniferyl aldehyde pH 8.0, 30°C Streptomyces sp.
134
-
NAD+ pH 8.0, 30°C Streptomyces sp.
536
-
coniferyl alcohol pH 8.0, 30°C Streptomyces sp.